{"id":"a011fc71-5fd5-59cc-a9ae-194f664bcc27","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:p6c-plp-trapping","predicate":"inactivates","statement":"Accumulated P6C reacts with PLP through Knoevenagel condensation, reducing cofactor availability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5a215d2a-737b-50e9-9cf7-847ee92ca712","mechanism_event_label":"A lysine metabolite can trap active vitamin B6.","subject":{"id":"9a7b1d83-ebb2-5dea-b4a7-c040502fba4d","slug":"piperideine-6-carboxylate","display_name":"Delta1-piperideine-6-carboxylate","entity_type_key":"small_molecule"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5a215d2a-737b-50e9-9cf7-847ee92ca712","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:p6c-plp-trapping-event","event_type":"biochemical_relationship","label":"A lysine metabolite can trap active vitamin B6.","description":"Accumulated P6C reacts with PLP through Knoevenagel condensation, reducing cofactor availability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9a7b1d83-ebb2-5dea-b4a7-c040502fba4d","slug":"piperideine-6-carboxylate","display_name":"Delta1-piperideine-6-carboxylate","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b5f7ef84-a782-50e1-8fdd-5ece73634310","slug":"p6c-plp-adduct","display_name":"P6C-PLP condensation product","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"affected_machinery","value_text":"ALDH7A1 upstream clearance","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"deficiency_not_equivalent","value_text":"Dietary lysine or vitamin B6 deficiency","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chemical mechanism associated with human ALDH7A1 disease","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Secondary cofactor loss does not demonstrate primary dietary vitamin B6 or lysine deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A lysine metabolite can trap active vitamin B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mills2006] Mutations in antiquitin in individuals with pyridoxine-dependent seizures. (2006). https://pubmed.ncbi.nlm.nih.gov/16491085/ DOI: 10.1038/nm1366","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"PDE-ALDH7A1 biochemical context","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1f6a3e60-7605-5059-8515-0f56f7db7194","evidence_kind":"source_excerpt","locator":"Lines 266-276","start_line":266,"end_line":276,"excerpt":"### p6c-plp-trapping\nAccumulated P6C reacts with PLP through Knoevenagel condensation, reducing cofactor availability.\nPlain language: A lysine metabolite can trap active vitamin B6.\nCondition category: machinery_impairment\norganism: Homo sapiens\ntissue_or_cell_type: PDE-ALDH7A1 biochemical context\nexperimental_model: Chemical mechanism associated with human ALDH7A1 disease\nlimitations: Secondary cofactor loss does not demonstrate primary dietary vitamin B6 or lysine deficiency.\naffected_machinery: ALDH7A1 upstream clearance\ndeficiency_not_equivalent: Dietary lysine or vitamin B6 deficiency\n[mills2006] Mutations in antiquitin in individuals with pyridoxine-dependent seizures. (2006). https://pubmed.ncbi.nlm.nih.gov/16491085/ DOI: 10.1038/nm1366","model_system":"Chemical mechanism associated with human ALDH7A1 disease","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [mills2006] Mutations in antiquitin in individuals with pyridoxine-dependent seizures. (2006). https://pubmed.ncbi.nlm.nih.gov/16491085/ DOI: 10.1038/nm1366","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}