{"id":"9fd32a8a-fba0-5947-a366-ceb5f95c9df6","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-knockout-tissue-zinc","predicate":"decreases","statement":"Enterocyte-specific Slc39a4 deletion rapidly lowered total zinc in mouse small intestine, liver and pancreas.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"bf06b0ba-7892-5e9c-9808-ce2c1a7d8278","mechanism_event_label":"Disabling intestinal ZIP4 depleted zinc beyond the intestine.","subject":{"id":"b704eea7-d5f4-577d-8801-1dd4e946623c","slug":"mouse-enterocyte-slc39a4-knockout","display_name":"Mouse enterocyte-specific Slc39a4 knockout genotype","entity_type_key":"gene"},"object":{"id":"4c429089-ed70-5d1f-93b5-386fa89c8674","slug":"tissue-total-zinc","display_name":"Tissue total zinc content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bf06b0ba-7892-5e9c-9808-ce2c1a7d8278","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-knockout-tissue-zinc-event","event_type":"biochemical_relationship","label":"Disabling intestinal ZIP4 depleted zinc beyond the intestine.","description":"Enterocyte-specific Slc39a4 deletion rapidly lowered total zinc in mouse small intestine, liver and pancreas.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b2399154-5b80-5772-bf7c-d9d80aefb691","slug":"mouse-slc39a4","display_name":"Mouse ZIP4 (Slc39a4)","entity_type_key":"protein"},"role":"disrupted_transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"measured_elemental_pool","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b704eea7-d5f4-577d-8801-1dd4e946623c","slug":"mouse-enterocyte-slc39a4-knockout","display_name":"Mouse enterocyte-specific Slc39a4 knockout genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4c429089-ed70-5d1f-93b5-386fa89c8674","slug":"tissue-total-zinc","display_name":"Tissue total zinc content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Tamoxifen-inducible enterocyte-specific knockout and tissue elemental analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Conditional gene deletion compared with intact controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A genetic transport defect is not interchangeable with low intake. Total tissue zinc does not resolve labile versus protein-bound zinc.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Disabling intestinal ZIP4 depleted zinc beyond the intestine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-22737083] A mouse model of acrodermatitis enteropathica: loss of intestine zinc transporter ZIP4 (Slc39a4) disrupts the stem cell niche and intestine integrity. (2012). https://pubmed.ncbi.nlm.nih.gov/22737083/ DOI: 10.1371/journal.pgen.1002766","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Small intestine, liver, pancreas","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"57364f47-e526-5f5c-b6fe-37aaff27f2b9","evidence_kind":"source_excerpt","locator":"Lines 271-282","start_line":271,"end_line":282,"excerpt":"### zinc-trans-zip4-knockout-tissue-zinc\nEnterocyte-specific Slc39a4 deletion rapidly lowered total zinc in mouse small intestine, liver and pancreas.\nCondition category: machinery_impairment\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Disabling intestinal ZIP4 depleted zinc beyond the intestine.\norganism: Mus musculus\ntissue_or_cell_type: Small intestine, liver, pancreas\nexperimental_model: Tamoxifen-inducible enterocyte-specific knockout and tissue elemental analysis\nlimitations: A genetic transport defect is not interchangeable with low intake. Total tissue zinc does not resolve labile versus protein-bound zinc.\nexposure: Conditional gene deletion compared with intact controls.\ncross_nutrient: false\n[zinc-trans-22737083] A mouse model of acrodermatitis enteropathica: loss of intestine zinc transporter ZIP4 (Slc39a4) disrupts the stem cell niche and intestine integrity. (2012). https://pubmed.ncbi.nlm.nih.gov/22737083/ DOI: 10.1371/journal.pgen.1002766","model_system":"Tamoxifen-inducible enterocyte-specific knockout and tissue elemental analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-22737083] A mouse model of acrodermatitis enteropathica: loss of intestine zinc transporter ZIP4 (Slc39a4) disrupts the stem cell niche and intestine integrity. (2012). https://pubmed.ncbi.nlm.nih.gov/22737083/ DOI: 10.1371/journal.pgen.1002766","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}