{"id":"9fcaf6da-13cc-557e-9c99-2b1826b38409","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-pde4","predicate":"inhibits","statement":"Resveratrol inhibited the tested recombinant PDE4 preparation with approximate IC50 14 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"56998504-3987-59b4-bf28-802ddccc4c79","mechanism_event_label":"Slower cAMP breakdown can extend a signaling pulse.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"60de6e73-590b-5479-8892-b396d05515dc","slug":"park-recombinant-pde4-preparation","display_name":"Recombinant PDE4 preparation in Park 2012","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"56998504-3987-59b4-bf28-802ddccc4c79","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-pde4-event","event_type":"observed_relationship","label":"Slower cAMP breakdown can extend a signaling pulse.","description":"Resveratrol inhibited the tested recombinant PDE4 preparation with approximate IC50 14 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"60de6e73-590b-5479-8892-b396d05515dc","slug":"park-recombinant-pde4-preparation","display_name":"Recombinant PDE4 preparation in Park 2012","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cc3323e-3a7f-5be5-91ae-78dab284fb81","slug":"cyclic-amp","display_name":"Cyclic adenosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free cyclic-nucleotide hydrolysis assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Retains preparation identity: do not infer an unreported isoform/species or a human effective dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Slower cAMP breakdown can extend a signaling pulse.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22304913/ · DOI 10.1016/j.cell.2012.01.017","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9eb3a5db-8bc4-55dd-8c13-5f2552fff60a","evidence_kind":"source_excerpt","locator":"Lines 222-228","start_line":222,"end_line":228,"excerpt":"## resveratrol-pde4\nSlower cAMP breakdown can extend a signaling pulse.\nResveratrol inhibited the tested recombinant PDE4 preparation with approximate IC50 14 micromolar.\nModel: Cell-free cyclic-nucleotide hydrolysis assay.\nLimitations: Retains preparation identity: do not infer an unreported isoform/species or a human effective dose.\nEvidence access: Primary full text\nResveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22304913/ · DOI 10.1016/j.cell.2012.01.017","model_system":"Cell-free cyclic-nucleotide hydrolysis assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"04885b4bee72f912a7f3f618f1b2bf79c944dbcb20500398c2729fc8228dcd9a","revision_id":"99504ff2-f1d3-5f4c-bc31-e1030d27a918","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}