{"id":"9fc41a10-2e75-5c2e-a494-5e5cf3275ad7","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-colon-gsr-association","predicate":"was_associated_with","statement":"The derivative-dependent ROS results tracked GSR protein levels in HT-29 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c2122363-601a-5098-88e0-0400f4aaf695","mechanism_event_label":"This identifies a shared enzyme connection without proving it caused the response.","subject":{"id":"892f07aa-be61-5b10-8b42-fba82999211c","slug":"colon-cell-ros-readout","display_name":"ROS readout in HT-29 and HCT-116 cells","entity_type_key":"cellular_process"},"object":{"id":"586ea0bf-ac47-5f0f-a039-77f90e7dfcc9","slug":"human-gsr-protein-abundance","display_name":"Human glutathione reductase protein abundance","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c2122363-601a-5098-88e0-0400f4aaf695","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-colon-gsr-association-event","event_type":"biochemical_relationship","label":"This identifies a shared enzyme connection without proving it caused the response.","description":"The derivative-dependent ROS results tracked GSR protein levels in HT-29 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae04c12f-c0ac-5c8d-9ee2-76e58156d105","slug":"gsr","display_name":"Glutathione reductase / GSR","entity_type_key":"protein"},"role":"measured_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"892f07aa-be61-5b10-8b42-fba82999211c","slug":"colon-cell-ros-readout","display_name":"ROS readout in HT-29 and HCT-116 cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"586ea0bf-ac47-5f0f-a039-77f90e7dfcc9","slug":"human-gsr-protein-abundance","display_name":"Human glutathione reductase protein abundance","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/nasunin-research/25442541.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"14f1cfb323570e36200958bb6acb4c083621b7b02ebda1e793d133b3ea95b1c7\", \"start_char\": 0, \"end_char\": 1299, \"text_sha256\": \"14f1cfb323570e36200958bb6acb4c083621b7b02ebda1e793d133b3ea95b1c7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Comparative anthocyanin exposure in cultured colon cancer lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Nasunin and differently glycosylated delphinidin derivatives; quantitative regimens not specified in indexed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary experiments are described despite an additional Review index tag. Protein association does not establish GSR-mediated causality; these are cancer cells, not normal colon tissue or patients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Nasunin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HT-29 and HCT-116 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This identifies a shared enzyme connection without proving it caused the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nasunin-p25442541] Effect of glycosylation patterns of Chinese eggplant anthocyanins and other derivatives on antioxidant effectiveness in human colon cell lines. (2015). https://pubmed.ncbi.nlm.nih.gov/25442541/ DOI: 10.1016/j.foodchem.2014.08.100","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"ROS, glutathione reductase protein and DNA damage","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"637814cf-e63e-5216-ae30-a9468cd59878","evidence_kind":"source_excerpt","locator":"Lines 692-703","start_line":692,"end_line":703,"excerpt":"### nasunin-colon-gsr-association\nThe derivative-dependent ROS results tracked GSR protein levels in HT-29 cells.\nCondition category: normal\nnutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This identifies a shared enzyme connection without proving it caused the response.\norganism: Human HT-29 and HCT-116 cells\ntissue_or_cell_type: ROS, glutathione reductase protein and DNA damage\nexperimental_model: Comparative anthocyanin exposure in cultured colon cancer lines\nlimitations: Primary experiments are described despite an additional Review index tag. Protein association does not establish GSR-mediated causality; these are cancer cells, not normal colon tissue or patients.\nexposure: Nasunin and differently glycosylated delphinidin derivatives; quantitative regimens not specified in indexed abstract\nevidence_span: {\"source_cache\": \"artifacts/nasunin-research/25442541.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"14f1cfb323570e36200958bb6acb4c083621b7b02ebda1e793d133b3ea95b1c7\", \"start_char\": 0, \"end_char\": 1299, \"text_sha256\": \"14f1cfb323570e36200958bb6acb4c083621b7b02ebda1e793d133b3ea95b1c7\"}\n[nasunin-p25442541] Effect of glycosylation patterns of Chinese eggplant anthocyanins and other derivatives on antioxidant effectiveness in human colon cell lines. 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