{"id":"9fbfd1ae-4f1d-5cc1-87fa-b5e0f319443d","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:rat-opc-tunel","predicate":"no_detected_change_in_recorded_experiment","statement":"SA did not significantly change the rat-opc-tunel readout.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"98e87ce5-e53f-576a-81f7-ba2636710587","mechanism_event_label":"SA did not significantly change the rat-opc-tunel readout.","subject":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"object":{"id":"81eff8ad-cf3b-5207-ae58-05ec3700b719","slug":"rat-opc-tunel","display_name":"Rat OPC TUNEL labeling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"98e87ce5-e53f-576a-81f7-ba2636710587","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:rat-opc-tunel-event","event_type":"experimental_observation","label":"SA did not significantly change the rat-opc-tunel readout.","description":"**Oligodendrocyte differentiation differs from proliferation.** In primary rat oligodendrocyte precursor cultures, shikimic acid at 1–100 µg/mL increased MBP-associated differentiation after 72 h, with 100 µg/mL used for subsequent experiments. That highest concentration is calculated as about 574 µM. BrdU incorporation and TUNEL readouts did not significantly change. Increased MBP and fewer NG2-positive cells therefore support maturation in this model, not universal stimulation of cell proliferation. [Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice.](https://pubmed.ncbi.nlm.nih.gov/30684125/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"SA 100 µg/mL","sequence_order":0,"notes":""},{"entity":{"id":"81eff8ad-cf3b-5207-ae58-05ec3700b719","slug":"rat-opc-tunel","display_name":"Rat OPC TUNEL labeling","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. 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The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SA did not significantly change the rat-opc-tunel readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice. | 2019 | DOI 10.1007/s12264-018-0322-7 | PMID 30684125 | https://pubmed.ncbi.nlm.nih.gov/30684125/ | https://doi.org/10.1007/s12264-018-0322-7 | https://pmc.ncbi.nlm.nih.gov/articles/PMC6527532/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 69-69; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e5ed3669-4bba-5759-93cf-bd443abf03ac","evidence_kind":"source_excerpt","locator":"Lines 69-69","start_line":69,"end_line":69,"excerpt":"**Oligodendrocyte differentiation differs from proliferation.** In primary rat oligodendrocyte precursor cultures, shikimic acid at 1–100 µg/mL increased MBP-associated differentiation after 72 h, with 100 µg/mL used for subsequent experiments. That highest concentration is calculated as about 574 µM. BrdU incorporation and TUNEL readouts did not significantly change. Increased MBP and fewer NG2-positive cells therefore support maturation in this model, not universal stimulation of cell proliferation. [Shikimic Acid Promotes Oligodendrocyte Precursor Cell Differentiation and Accelerates Remyelination in Mice.](https://pubmed.ncbi.nlm.nih.gov/30684125/)","model_system":"Primary rat OPCs, 100 µg/mL SA versus vehicle.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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