{"id":"9f86d73e-7daa-5ed3-ad0f-10706d00bb6d","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-brain-luminal-localization","predicate":"preferentially_localizes_to","statement":"The brain-microvessel study localized SLC5A6 preferentially to the luminal membrane of brain capillary endothelium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ca9edbf4-6e1d-5958-a1fd-4797bf8c3cb3","mechanism_event_label":"Brain capillary SMVT is concentrated on the side facing the blood.","subject":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"object":{"id":"67dc2fa0-f0ad-57be-8821-7176ed7d862f","slug":"brain-capillary-luminal-membrane","display_name":"Brain capillary luminal membrane","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"ca9edbf4-6e1d-5958-a1fd-4797bf8c3cb3","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-brain-luminal-localization-event","event_type":"biochemical_relationship","label":"Brain capillary SMVT is concentrated on the side facing the blood.","description":"The brain-microvessel study localized SLC5A6 preferentially to the luminal membrane of brain capillary endothelium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"transport_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"transport_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3bd59e38-e6b1-5223-b4de-faa33835f5bc","slug":"slc5a6","display_name":"Human sodium-dependent multivitamin transporter / SLC5A6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"67dc2fa0-f0ad-57be-8821-7176ed7d862f","slug":"brain-capillary-luminal-membrane","display_name":"Brain capillary luminal membrane","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human and monkey brain microvessel analyses; SLC5A6-siRNA experiments in human hCMEC/D3 endothelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Antibody-free membrane-localization method, with human/monkey microvessel proteomics and human expression analyses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract does not resolve which species supplied every localization preparation. The result addresses blood-facing localization, not the unidentified abluminal export step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens and monkey microvessel preparations; localization sample allocation not detailed in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Brain capillary SMVT is concentrated on the side facing the blood.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-brain2015] Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. (2015). https://pubmed.ncbi.nlm.nih.gov/25809983/ DOI: 10.1111/jnc.13092","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain capillary endothelium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d6e9344e-2d73-5ecd-a633-30122115afa5","evidence_kind":"source_excerpt","locator":"Lines 301-312","start_line":301,"end_line":312,"excerpt":"### b5-trans-brain-luminal-localization\nThe brain-microvessel study localized SLC5A6 preferentially to the luminal membrane of brain capillary endothelium.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Brain capillary SMVT is concentrated on the side facing the blood.\norganism: Homo sapiens and monkey microvessel preparations; localization sample allocation not detailed in abstract\ntissue_or_cell_type: Brain capillary endothelium\nexperimental_model: Human and monkey brain microvessel analyses; SLC5A6-siRNA experiments in human hCMEC/D3 endothelial cells\nlimitations: Abstract does not resolve which species supplied every localization preparation. The result addresses blood-facing localization, not the unidentified abluminal export step.\nexposure: Antibody-free membrane-localization method, with human/monkey microvessel proteomics and human expression analyses.\ncross_nutrient: true\n[b5-trans-brain2015] Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. (2015). https://pubmed.ncbi.nlm.nih.gov/25809983/ DOI: 10.1111/jnc.13092","model_system":"Human and monkey brain microvessel analyses; SLC5A6-siRNA experiments in human hCMEC/D3 endothelial cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-brain2015] Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. 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