{"id":"9f81de12-981b-55ae-a27d-2422ddcfb0ce","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-adams-local-conversion","predicate":"supports-local-production-of","statement":"Adding 100 nM 25(OH)D3 increased detectable 1, 25(OH)2D in conditioned monocyte medium even without TLR induction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8145ed9c-84fc-52c8-8950-09e628cad420","mechanism_event_label":"These immune cells could activate the precursor locally.","subject":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"object":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8145ed9c-84fc-52c8-8950-09e628cad420","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-adams-local-conversion-event","event_type":"biochemical_relationship","label":"These immune cells could activate the precursor locally.","description":"Adding 100 nM 25(OH)D3 increased detectable 1, 25(OH)2D in conditioned monocyte medium even without TLR induction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a1d0238c-7529-5175-b5f9-71dc9bc61ed5","slug":"cyp27b1","display_name":"Vitamin D 1-alpha-hydroxylase / CYP27B1","entity_type_key":"protein"},"role":"activation-enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6bd73bd2-1f0a-5caa-ba69-7ce1b75364bb","slug":"calcifediol","display_name":"Calcifediol / 25-hydroxyvitamin D3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f5156c24-108f-5746-b114-ed764e79a3f4","slug":"calcitriol","display_name":"Calcitriol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Human monocytes, 24 hours, 100 nMadded 25(OH)D3; Fig 3C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex-vivo monocytes in 10% serum, not a whole-person infection endpoint. Low-substrate medium is not a validated universal blood threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"These immune cells could activate the precursor locally.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. (2009). https://pubmed.ncbi.nlm.nih.gov/19299728/ DOI: 10.4049/jimmunol.0803736","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human monocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"09c8194f-c6fb-5239-b78d-13ee75bfbd77","evidence_kind":"source_excerpt","locator":"Lines 996-1007","start_line":996,"end_line":1007,"excerpt":"### vd-adams-local-conversion\nAdding 100 nM 25(OH)D3 increased detectable 1, 25(OH)2D in conditioned monocyte medium even without TLR induction.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: These immune cells could activate the precursor locally.\norganism: Homo sapiens\ntissue_or_cell_type: Human monocytes\nexperimental_model: Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum\nlimitations: Ex-vivo monocytes in 10% serum, not a whole-person infection endpoint. Low-substrate medium is not a validated universal blood threshold.\nexposure: Human monocytes, 24 hours, 100 nMadded 25(OH)D3; Fig 3C.\ncross_nutrient: false\n[adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. (2009). https://pubmed.ncbi.nlm.nih.gov/19299728/ DOI: 10.4049/jimmunol.0803736","model_system":"Human bone-clinic cohort and ex-vivo monocytes in 10% autologous serum","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [adams2009] Vitamin d-directed rheostatic regulation of monocyte antibacterial responses. 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