{"id":"9f796c76-bd7b-5415-a192-f0288577107d","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-tf-uptake-reduction","predicate":"reduces","statement":"Transferrin binding reduced vanadium uptake and antiproliferative activity in human A549 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"40226e3a-7e97-5d82-8566-62de384f4350","mechanism_event_label":"Binding to a carrier protein can reduce delivery rather than improve it.","subject":{"id":"bf79be92-557a-54b3-8f78-7174ed04675d","slug":"human-vanadium-bound-transferrin","display_name":"Human transferrin bound to vanadium(V/IV)","entity_type_key":"protein_state"},"object":{"id":"859a35b0-1438-582e-a0a2-f956d6b0bb27","slug":"human-a549-vanadium-uptake","display_name":"Vanadium uptake in human A549 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"40226e3a-7e97-5d82-8566-62de384f4350","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-tf-uptake-reduction-event","event_type":"observed_relationship","label":"Binding to a carrier protein can reduce delivery rather than improve it.","description":"Transferrin binding reduced vanadium uptake and antiproliferative activity in human A549 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf79be92-557a-54b3-8f78-7174ed04675d","slug":"human-vanadium-bound-transferrin","display_name":"Human transferrin bound to vanadium(V/IV)","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"859a35b0-1438-582e-a0a2-f956d6b0bb27","slug":"human-a549-vanadium-uptake","display_name":"Vanadium uptake in human A549 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"656eb223-933b-5a40-b70c-0d77537fe717","slug":"vanadyl-iv","display_name":"Oxovanadium(IV) / vanadyl","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"05415b89-a435-5940-a9ba-b587a0b6f04d","slug":"tfrc","display_name":"Human transferrin receptor 1 / TFRC","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human lung-cancer cell uptake and proliferation assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish every tissue’s uptake route or clinical efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Binding to a carrier protein can reduce delivery rather than improve it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadium(V/IV)-Transferrin Binding Disrupts the Transferrin Cycle and Reduces Vanadium Uptake and Antiproliferative Activity in Human Lung Cancer Cells. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32578416/ · DOI 10.1021/acs.inorgchem.0c00926","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4743ec1c-fcb7-50c8-ae7c-457dba120403","evidence_kind":"source_excerpt","locator":"Lines 198-204","start_line":198,"end_line":204,"excerpt":"## vanadium-tf-uptake-reduction\nBinding to a carrier protein can reduce delivery rather than improve it.\nTransferrin binding reduced vanadium uptake and antiproliferative activity in human A549 cells.\nModel: Human lung-cancer cell uptake and proliferation assays.\nLimitations: Does not establish every tissue’s uptake route or clinical efficacy.\nEvidence access: Primary abstract\nVanadium(V/IV)-Transferrin Binding Disrupts the Transferrin Cycle and Reduces Vanadium Uptake and Antiproliferative Activity in Human Lung Cancer Cells. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32578416/ · DOI 10.1021/acs.inorgchem.0c00926","model_system":"Human lung-cancer cell uptake and proliferation assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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