{"id":"9e8f9598-ccb1-5b9d-80d5-dc3d9f338a53","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-mg-trapping","predicate":"reduces","statement":"Adding carnosine inhibited methylglyoxal-driven protein modification in electrophoretic assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"95ef27e0-73ce-5531-bda7-c088301ff36b","mechanism_event_label":"Another reactive carbonyl had less opportunity to modify proteins.","subject":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"object":{"id":"716be4f8-5b0f-5bed-a6f1-62b7f0627d3a","slug":"cell-free-mg-protein-modification","display_name":"Cell-free methylglyoxal-mediated protein modification","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"95ef27e0-73ce-5531-bda7-c088301ff36b","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-mg-trapping-event","event_type":"observed_relationship","label":"Another reactive carbonyl had less opportunity to modify proteins.","description":"Adding carnosine inhibited methylglyoxal-driven protein modification in electrophoretic assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"716be4f8-5b0f-5bed-a6f1-62b7f0627d3a","slug":"cell-free-mg-protein-modification","display_name":"Cell-free methylglyoxal-mediated protein modification","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"55c17a25-868a-5496-a200-88d9ec014558","slug":"methylglyoxal","display_name":"Methylglyoxal","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free protein incubation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Clinical glucose lowering is not established by this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Another reactive carbonyl had less opportunity to modify proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnosine protects proteins against methylglyoxal-mediated modifications. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9675080/ · DOI 10.1006/bbrc.1998.8806","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0a8e995b-27db-5786-9a54-686d005857ad","evidence_kind":"source_excerpt","locator":"Lines 252-258","start_line":252,"end_line":258,"excerpt":"## carnosine-mg-trapping\nAnother reactive carbonyl had less opportunity to modify proteins.\nAdding carnosine inhibited methylglyoxal-driven protein modification in electrophoretic assays.\nModel: Cell-free protein incubation.\nLimitations: Clinical glucose lowering is not established by this assay.\nEvidence access: Primary abstract\nCarnosine protects proteins against methylglyoxal-mediated modifications. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9675080/ · DOI 10.1006/bbrc.1998.8806","model_system":"Cell-free protein incubation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"469cbc38203db56bd031f9d8a2dd2bfa387713950ca30199511cd137e927aa7a","revision_id":"cc409a15-8b04-50ee-a7ff-d1bf32f686bb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}