{"id":"9e79b4ae-8532-5174-b55d-a10a8cb7bcac","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-impa-magnesium","predicate":"required_for","statement":"Mg2+ was essential for human inositol monophosphatase activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"166f18f2-e72b-5dd1-8e94-2ce3fefdb7a3","mechanism_event_label":"Inositol recycling needs magnesium at the enzyme level.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"3f404867-317e-5b5c-92a7-9b6e0411dec3","slug":"impa1","display_name":"IMPA1 (human inositol monophosphatase 1)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"166f18f2-e72b-5dd1-8e94-2ce3fefdb7a3","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-impa-magnesium-event","event_type":"biochemical_relationship","label":"Inositol recycling needs magnesium at the enzyme level.","description":"Mg2+ was essential for human inositol monophosphatase activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f404867-317e-5b5c-92a7-9b6e0411dec3","slug":"impa1","display_name":"IMPA1 (human inositol monophosphatase 1)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/8223565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\", \"start_char\": 0, \"end_char\": 1621, \"text_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Site-directed mutagenesis and enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Magnesium and lithium titrations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical concentration dependence; neither lithium treatment mechanism nor magnesium supplementation response is established by this assay alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human recombinant protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Inositol recycling needs magnesium at the enzyme level.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p8223565] Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis. (1993). https://pubmed.ncbi.nlm.nih.gov/8223565/ DOI: 10.1111/j.1432-1033.1993.tb18244.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified IMPA1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1acfde9a-5494-5c88-ac08-4f721001782a","evidence_kind":"source_excerpt","locator":"Lines 249-260","start_line":249,"end_line":260,"excerpt":"### ino-impa-magnesium\nMg2+ was essential for human inositol monophosphatase activity.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Inositol recycling needs magnesium at the enzyme level.\norganism: Human recombinant protein\ntissue_or_cell_type: Purified IMPA1\nexperimental_model: Site-directed mutagenesis and enzyme kinetics\nlimitations: Biochemical concentration dependence; neither lithium treatment mechanism nor magnesium supplementation response is established by this assay alone.\nexposure: Magnesium and lithium titrations\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/8223565.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\", \"start_char\": 0, \"end_char\": 1621, \"text_sha256\": \"945d1d5166ce6410cd69984d93aebf127b635dcfcebddba31f0e8cc8b1c304a5\"}\n[ino-p8223565] Probing the role of metal ions in the mechanism of inositol monophosphatase by site-directed mutagenesis. 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