{"id":"9e18a6d8-5c6c-5b90-9d82-755e04487b46","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-efflux-nek7-assembly","predicate":"supports","statement":"NEK7 loss blocked inflammasome assembly downstream of preserved K efflux; 50 mM KCl prevented the induced NEK7-NLRP3 interaction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ddb81aa6-0ec8-5fc3-b625-3b9e9c9dfb79","mechanism_event_label":"Potassium loss signals through an assembly protein before caspase activation.","subject":{"id":"bb8b5022-adbd-5231-b0d9-6a654e89ba6c","slug":"nek7","display_name":"NIMA-related kinase 7 / NEK7","entity_type_key":"protein"},"object":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"ddb81aa6-0ec8-5fc3-b625-3b9e9c9dfb79","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-efflux-nek7-assembly-event","event_type":"biochemical_relationship","label":"Potassium loss signals through an assembly protein before caspase activation.","description":"NEK7 loss blocked inflammasome assembly downstream of preserved K efflux; 50 mM KCl prevented the induced NEK7-NLRP3 interaction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b53e832d-0776-5495-b5cd-cb6a8e8c538b","slug":"nlrp3","display_name":"NLRP3","entity_type_key":"protein"},"role":"binding partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d4f83161-d4b0-5b91-b3ed-84d44d2b2bae","slug":"pycard","display_name":"ASC / PYCARD","entity_type_key":"protein"},"role":"assembly adaptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b8161b1e-3f89-55ee-b2c2-5aa83ed0d9ff","slug":"casp1","display_name":"Caspase-1 / CASP1","entity_type_key":"protein"},"role":"activation endpoint","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"upstream efflux signal","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bb8b5022-adbd-5231-b0d9-6a654e89ba6c","slug":"nek7","display_name":"NIMA-related kinase 7 / NEK7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Mouse macrophages with Nek7 deletion/reconstitution; ATP, nigericin or gramicidin; high-K control, interaction assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"NEK7 catalytic activity was dispensable; this is not proof of potassium binding directly to NEK7.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Potassium loss signals through an assembly protein before caspase activation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[he-2016-nek7] NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC4810788/ DOI: 10.1038/nature16959","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bone-marrow macrophages","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9a6cebfc-c9c4-54fc-839f-6a8a6d02b941","evidence_kind":"source_excerpt","locator":"Lines 880-889","start_line":880,"end_line":889,"excerpt":"### k-efflux-nek7-assembly\nNEK7 loss blocked inflammasome assembly downstream of preserved K efflux; 50 mM KCl prevented the induced NEK7-NLRP3 interaction.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Potassium loss signals through an assembly protein before caspase activation.\norganism: Mouse\ntissue_or_cell_type: Bone-marrow macrophages\nexperimental_model: Mouse macrophages with Nek7 deletion/reconstitution; ATP, nigericin or gramicidin; high-K control, interaction assays.\nlimitations: NEK7 catalytic activity was dispensable; this is not proof of potassium binding directly to NEK7.\n[he-2016-nek7] NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC4810788/ DOI: 10.1038/nature16959","model_system":"Mouse macrophages with Nek7 deletion/reconstitution; ATP, nigericin or gramicidin; high-K control, interaction assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [he-2016-nek7] NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux (2016). https://pmc.ncbi.nlm.nih.gov/articles/PMC4810788/ DOI: 10.1038/nature16959","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}