{"id":"9df5446f-7ce7-51cf-afec-567ff1b01d4f","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-carnmt1-protein","predicate":"methylates","statement":"CARNMT1 methylated histidine-containing C3H zinc-finger substrates including U2AF1; cellular mass spectrometry supported these modifications.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fc0a1ad2-d71c-567b-b650-e0f033ca3cd5","mechanism_event_label":"The carnosine methylating enzyme also acts on proteins.","subject":{"id":"02c057af-ec05-545c-8bcb-73ef7b9b3401","slug":"human-carnmt1","display_name":"Human carnosine N-methyltransferase / CARNMT1","entity_type_key":"protein"},"object":{"id":"cfc0f303-f94e-5bc4-9c56-4b437a48cf9a","slug":"human-u2af1","display_name":"Human splicing factor U2AF1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fc0a1ad2-d71c-567b-b650-e0f033ca3cd5","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-carnmt1-protein-event","event_type":"observed_relationship","label":"The carnosine methylating enzyme also acts on proteins.","description":"CARNMT1 methylated histidine-containing C3H zinc-finger substrates including U2AF1; cellular mass spectrometry supported these modifications.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"02c057af-ec05-545c-8bcb-73ef7b9b3401","slug":"human-carnmt1","display_name":"Human carnosine N-methyltransferase / CARNMT1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cfc0f303-f94e-5bc4-9c56-4b437a48cf9a","slug":"human-u2af1","display_name":"Human splicing factor U2AF1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f6954a9f-8df9-5bc1-9813-29f5f40e9dad","slug":"human-methylated-u2af1","display_name":"Human histidine-methylated U2AF1","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cellular extracts, recombinant-enzyme assays and mass spectrometry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not show that carnosine supplements alter RNA processing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The carnosine methylating enzyme also acts on proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of substrates and sequence requirements for CARNMT1-mediated histidine methylation of C3H zinc fingers. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40473212/ · DOI 10.1016/j.jbc.2025.110335","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c7ecd4a2-3e3d-546a-947d-96b4488b6633","evidence_kind":"source_excerpt","locator":"Lines 156-162","start_line":156,"end_line":162,"excerpt":"## carnosine-carnmt1-protein\nThe carnosine methylating enzyme also acts on proteins.\nCARNMT1 methylated histidine-containing C3H zinc-finger substrates including U2AF1; cellular mass spectrometry supported these modifications.\nModel: Human cellular extracts, recombinant-enzyme assays and mass spectrometry.\nLimitations: This does not show that carnosine supplements alter RNA processing.\nEvidence access: Primary abstract\nIdentification of substrates and sequence requirements for CARNMT1-mediated histidine methylation of C3H zinc fingers. · 2025 · https://pubmed.ncbi.nlm.nih.gov/40473212/ · DOI 10.1016/j.jbc.2025.110335","model_system":"Human cellular extracts, recombinant-enzyme assays and mass spectrometry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"469cbc38203db56bd031f9d8a2dd2bfa387713950ca30199511cd137e927aa7a","revision_id":"cc409a15-8b04-50ee-a7ff-d1bf32f686bb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}