{"id":"9d854028-0e45-5eb5-b730-8abf180c7b8f","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-fmlp-null","predicate":"does_not_reduce","statement":"The same bromelain treatment did not reduce human neutrophil migration toward fMLP.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"6dca9142-8390-590b-b267-a45ee67591ee","mechanism_event_label":"The same bromelain treatment did not reduce human neutrophil migration toward fMLP.","subject":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"object":{"id":"7d3668c1-e0d6-59e9-a522-4b69d8d52e90","slug":"human-neutrophil-fmlp-chemotaxis","display_name":"Human neutrophil chemotaxis toward fMLP","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6dca9142-8390-590b-b267-a45ee67591ee","stable_key":"773f772e-bf8e-5e52-a2a2-6dd694d67f5d:bromelain-fmlp-null-event","event_type":"observed_relationship","label":"The same bromelain treatment did not reduce human neutrophil migration toward fMLP.","description":"The same bromelain treatment did not reduce human neutrophil migration toward fMLP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a3990e6-85af-58c7-8087-8a251db842fa","slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7d3668c1-e0d6-59e9-a522-4b69d8d52e90","slug":"human-neutrophil-fmlp-chemotaxis","display_name":"Human neutrophil chemotaxis toward fMLP","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"27f5dd6b-f9f8-525f-b961-51a1842640e0","slug":"fmlp","display_name":"N-formyl-methionyl-leucyl-phenylalanine / fMLP","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Bromelain pretreatment followed by fMLP","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human neutrophils","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This null result limits the mechanism to selected surface receptors; it does not prove every non-IL-8 chemotactic route is intact.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Bromelain chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"bromelain","display_name":"Bromelain","entity_type_key":"peptide"}},{"dimension":"organism","value_text":"Isolated human neutrophils","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same bromelain treatment did not reduce human neutrophil migration toward fMLP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bromelain treatment decreases neutrophil migration to sites of inflammation. (2008). https://pubmed.ncbi.nlm.nih.gov/18482869/ DOI: 10.1016/j.clim.2008.02.015","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Stimulus-specific chemotaxis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"058e6b73-aa25-538a-80d8-d82c39ca1781","evidence_kind":"source_excerpt","locator":"Lines 44-53","start_line":44,"end_line":53,"excerpt":"## bromelain-fmlp-null\nThe same bromelain treatment did not reduce human neutrophil migration toward fMLP.\nModel/species: Isolated human neutrophils\nTissue/system: Stimulus-specific chemotaxis\nExposure: Bromelain pretreatment followed by fMLP\nRoute: In vitro\nDuration: Acute\nLimits: This null result limits the mechanism to selected surface receptors; it does not prove every non-IL-8 chemotactic route is intact.\nPrimary reference: Bromelain treatment decreases neutrophil migration to sites of inflammation. (2008). https://pubmed.ncbi.nlm.nih.gov/18482869/ DOI: 10.1016/j.clim.2008.02.015\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Isolated human neutrophils","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9d363b4e-f438-5664-9d74-99c2e359391d","stable_key":"import-773f772e-bf8e-5e52-a2a2-6dd694d67f5d","title":"Bromelain: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"b328dbb8082bea612bb2c40d6ff41fa670f6e62f714bd014c90627fcab86e95b","revision_id":"1c10d22e-ecdf-56ef-b820-bb21182a9577","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}