{"id":"9d6b4fbe-6aef-5434-8c80-63f25730df8a","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-cyst-growth","predicate":"reduces","statement":"Steviol at 100 micromolar reversibly inhibited MDCK cyst formation and growth; doses up to 200 micromolar did not alter measured cell viability, proliferation or apoptosis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6dbf128d-0397-560f-a89c-47da4c65cdf7","mechanism_event_label":"Reduced fluid-secretion-associated growth was separable from measured cell killing in this assay.","subject":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"object":{"id":"dac7c14a-732b-5974-9d9a-e3b97bbee4da","slug":"dog-mdck-cyst-growth","display_name":"Canine MDCK cyst growth","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6dbf128d-0397-560f-a89c-47da4c65cdf7","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-cyst-growth-event","event_type":"observed_relationship","label":"Reduced fluid-secretion-associated growth was separable from measured cell killing in this assay.","description":"Steviol at 100 micromolar reversibly inhibited MDCK cyst formation and growth; doses up to 200 micromolar did not alter measured cell viability, proliferation or apoptosis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dac7c14a-732b-5974-9d9a-e3b97bbee4da","slug":"dog-mdck-cyst-growth","display_name":"Canine MDCK cyst growth","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b04e6b2-6fd0-5a0d-8f08-565c7f1e61cc","slug":"dog-cftr","display_name":"Canine cystic fibrosis transmembrane conductance regulator / CFTR","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Three-dimensional canine kidney cyst culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not show clinical treatment of human polycystic kidney disease.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Reduced fluid-secretion-associated growth was separable from measured cell killing in this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Steviol reduces MDCK Cyst formation and growth by inhibiting CFTR channel activity and promoting proteasome-mediated CFTR degradation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23536832/ · DOI 10.1371/journal.pone.0058871","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"14dc4094-3315-54d2-98a7-06b047595a1e","evidence_kind":"source_excerpt","locator":"Lines 314-320","start_line":314,"end_line":320,"excerpt":"## stevia-cyst-growth\nReduced fluid-secretion-associated growth was separable from measured cell killing in this assay.\nSteviol at 100 micromolar reversibly inhibited MDCK cyst formation and growth; doses up to 200 micromolar did not alter measured cell viability, proliferation or apoptosis.\nModel: Three-dimensional canine kidney cyst culture.\nLimitations: This does not show clinical treatment of human polycystic kidney disease.\nEvidence access: Primary abstract\nSteviol reduces MDCK Cyst formation and growth by inhibiting CFTR channel activity and promoting proteasome-mediated CFTR degradation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23536832/ · DOI 10.1371/journal.pone.0058871","model_system":"Three-dimensional canine kidney cyst culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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