{"id":"9d2de808-19f9-503f-b2b9-7cf907742e0f","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-ros-induction","predicate":"increases_tested","statement":"Aspartame increased the ROS-sensitive fluorescence signal in exposed Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4ff2dc99-be70-5c12-995b-41e4a24c317e","mechanism_event_label":"Oxidative signaling is an experimentally measured intermediate.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"afffe733-49d5-55e9-8895-6e5e78d0f534","slug":"human-caco2-aspartame-ros","display_name":"Human Caco-2 ROS signal during aspartame exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4ff2dc99-be70-5c12-995b-41e4a24c317e","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-ros-induction-event","event_type":"observed_relationship","label":"Oxidative signaling is an experimentally measured intermediate.","description":"Aspartame increased the ROS-sensitive fluorescence signal in exposed Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"afffe733-49d5-55e9-8895-6e5e78d0f534","slug":"human-caco2-aspartame-ros","display_name":"Human Caco-2 ROS signal during aspartame exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Figure 5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"0.1 mM, 24 hours; DCFDA assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Probe fluorescence does not identify a single radical species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Oxidative signaling is an experimentally measured intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Artificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"73a2d438-b72e-5cf8-8807-ae0758b91104","evidence_kind":"source_excerpt","locator":"Lines 218-224","start_line":218,"end_line":224,"excerpt":"## aspartame-ros-induction\nOxidative signaling is an experimentally measured intermediate.\nAspartame increased the ROS-sensitive fluorescence signal in exposed Caco-2 cells.\nModel: 0.1 mM, 24 hours; DCFDA assay.\nLimitations: Probe fluorescence does not identify a single radical species.\nEvidence access: Primary full text; Figure 5\nArtificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","model_system":"0.1 mM, 24 hours; DCFDA assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}