{"id":"9d0ba718-2bfb-543b-9ec3-c0feb87f377e","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-tgf-neutralization","predicate":"neutralization_abrogates","statement":"In-vivo neutralization of LAP-expressing cells abolished CLA-associated tumor promotion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"43c7b96d-5441-5e67-ae81-c3ab7202c64f","mechanism_event_label":"Perturbing the TGF-beta-associated cell population changed the result.","subject":{"id":"8c1ce843-8860-515c-bd7d-902529008e35","slug":"mouse-tgf-beta-producing-immune-cells","display_name":"Mouse TGF-beta-producing macrophages and T cells","entity_type_key":"cellular_process"},"object":{"id":"351f8854-621c-5287-9107-6637a331c128","slug":"mouse-aom-dss-tumor-burden","display_name":"Mouse AOM/DSS colorectal tumor burden","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"43c7b96d-5441-5e67-ae81-c3ab7202c64f","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-tgf-neutralization-event","event_type":"observed_relationship","label":"Perturbing the TGF-beta-associated cell population changed the result.","description":"In-vivo neutralization of LAP-expressing cells abolished CLA-associated tumor promotion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c1ce843-8860-515c-bd7d-902529008e35","slug":"mouse-tgf-beta-producing-immune-cells","display_name":"Mouse TGF-beta-producing macrophages and T cells","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"351f8854-621c-5287-9107-6637a331c128","slug":"mouse-aom-dss-tumor-burden","display_name":"Mouse AOM/DSS colorectal tumor burden","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"93f9c954-8c9b-531e-ab62-e9e267e43b6e","slug":"mouse-tgfb1","display_name":"Mouse transforming growth factor beta 1 / Tgfb1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse antibody-intervention experiment targeting latency-associated peptide-expressing cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not equivalent to proving that every TGF-beta signal was blocked or identifying a human treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Perturbing the TGF-beta-associated cell population changed the result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"CLA-supplemented diet accelerates experimental colorectal cancer by inducing TGF-β-producing macrophages and T cells. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30279515/ · DOI 10.1038/s41385-018-0090-8","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c57a6c74-fe77-5bd0-a778-af254fb69e71","evidence_kind":"source_excerpt","locator":"Lines 406-412","start_line":406,"end_line":412,"excerpt":"## cla-tgf-neutralization\nPerturbing the TGF-beta-associated cell population changed the result.\nIn-vivo neutralization of LAP-expressing cells abolished CLA-associated tumor promotion.\nModel: Mouse antibody-intervention experiment targeting latency-associated peptide-expressing cells.\nLimitations: Not equivalent to proving that every TGF-beta signal was blocked or identifying a human treatment.\nEvidence access: Primary abstract\nCLA-supplemented diet accelerates experimental colorectal cancer by inducing TGF-β-producing macrophages and T cells. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30279515/ · DOI 10.1038/s41385-018-0090-8","model_system":"Mouse antibody-intervention experiment targeting latency-associated peptide-expressing cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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