{"id":"9d0775b0-bc2f-533d-adf8-425277ed00c1","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-neutrophil-pde","predicate":"inhibits","statement":"Adding 6-gingerol reduced human neutrophil lysate cAMP-PDE activity by about 40 percent.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8c182b16-6c2c-5f5e-a9c2-5d1d758e0579","mechanism_event_label":"Adding 6-gingerol reduced human neutrophil lysate cAMP-PDE activity by about 40 percent.","subject":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"e82e8bb4-b6e0-5694-9e9a-10f3c19dddd2","slug":"human-neutrophil-camp-pde-activity","display_name":"cAMP phosphodiesterase activity in human neutrophil lysates","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8c182b16-6c2c-5f5e-a9c2-5d1d758e0579","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-neutrophil-pde-event","event_type":"observed_relationship","label":"Adding 6-gingerol reduced human neutrophil lysate cAMP-PDE activity by about 40 percent.","description":"Adding 6-gingerol reduced human neutrophil lysate cAMP-PDE activity by about 40 percent.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e82e8bb4-b6e0-5694-9e9a-10f3c19dddd2","slug":"human-neutrophil-camp-pde-activity","display_name":"cAMP phosphodiesterase activity in human neutrophil lysates","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cc3323e-3a7f-5be5-91ae-78dab284fb81","slug":"cyclic-amp","display_name":"Cyclic adenosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"6-Gingerol 10 micromolar; rolipram 0.1 micromolar comparator","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"1 h at 37 degrees C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary open full text, results, figure legends and methods, plus PubMed metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Lysates of human donor neutrophils","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mixed cAMP-PDE activity was measured; similarity to rolipram does not establish isoform selectivity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Lysates of human donor neutrophils","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding 6-gingerol reduced human neutrophil lysate cAMP-PDE activity by about 40 percent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antineutrophil properties of natural gingerols in models of lupus. (2021). https://pubmed.ncbi.nlm.nih.gov/33373329/ DOI: 10.1172/jci.insight.138385","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Ex vivo lysate assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Neutrophil enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8bcfc30b-585d-5ad9-95b2-751200ff4234","evidence_kind":"source_excerpt","locator":"Lines 448-457","start_line":448,"end_line":457,"excerpt":"## gingerols-6-neutrophil-pde\nAdding 6-gingerol reduced human neutrophil lysate cAMP-PDE activity by about 40 percent.\nModel/species: Lysates of human donor neutrophils\nTissue: Neutrophil enzyme preparation\nExposure: 6-Gingerol 10 micromolar; rolipram 0.1 micromolar comparator\nRoute: Ex vivo lysate assay\nDuration: 1 h at 37 degrees C\nLimits: Mixed cAMP-PDE activity was measured; similarity to rolipram does not establish isoform selectivity.\nPrimary reference: Antineutrophil properties of natural gingerols in models of lupus. (2021). https://pubmed.ncbi.nlm.nih.gov/33373329/ DOI: 10.1172/jci.insight.138385\nAccess: Primary open full text, results, figure legends and methods, plus PubMed metadata.","model_system":"Lysates of human donor neutrophils","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived synthesis span with its primary citation; original paraphrase, not a claimed publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1107d60b-8e2d-55d7-8f5d-5612c49aa7dc","stable_key":"import-fd7915ff-856d-56c2-a118-4989693a7644","title":"Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text.","file_path":"","sha256":"96d1b7b04445eecd0eac1738e0c80cb6308509ecdfc3b5fc6be67ae2218cbb2b","revision_id":"de6e70ff-0e52-5c26-bd3a-54fca2085a86","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}