{"id":"9d06e895-fe15-5322-bcd2-a3c3b13000e9","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-glycine-dopamine","predicate":"antagonism_attenuates_theanine_response_of","statement":"Strychnine reduced the theanine-induced dopamine response in rat striatum.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b2ab5b1d-40d3-582f-8fc5-57f42fdae812","mechanism_event_label":"The glycine-receptor pathway participated in the dopamine response.","subject":{"id":"665e1d09-4da7-5c90-8d26-9e4f1711a165","slug":"rat-glycine-receptors","display_name":"Rat striatal glycine receptors, subunits unresolved","entity_type_key":"protein_family"},"object":{"id":"f2f4d3ba-adf2-515b-a36d-b5472d839b61","slug":"striatal-extracellular-dopamine","display_name":"Rat striatal extracellular dopamine concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b2ab5b1d-40d3-582f-8fc5-57f42fdae812","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-glycine-dopamine-event","event_type":"biochemical_relationship","label":"The glycine-receptor pathway participated in the dopamine response.","description":"Strychnine reduced the theanine-induced dopamine response in rat striatum.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e1378422-bd3f-51e8-b487-a60e31a2b6a8","slug":"strychnine","display_name":"Strychnine","entity_type_key":"small_molecule"},"role":"antagonist","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cca6209-0603-5ab7-96ad-2f3ae4b772d2","slug":"dopamine","display_name":"Dopamine","entity_type_key":"small_molecule"},"role":"measured_transmitter","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"665e1d09-4da7-5c90-8d26-9e4f1711a165","slug":"rat-glycine-receptors","display_name":"Rat striatal glycine receptors, subunits unresolved","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f2f4d3ba-adf2-515b-a36d-b5472d839b61","slug":"striatal-extracellular-dopamine","display_name":"Rat striatal extracellular dopamine concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/18196445.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"59fdaf94d59c2f4b2981c29cd2981c043351aae1299a59a19e29979076957839\", \"start_char\": 0, \"end_char\": 1118, \"text_sha256\": \"59fdaf94d59c2f4b2981c29cd2981c043351aae1299a59a19e29979076957839\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In-vivo striatal microdialysis with receptor antagonists","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Local brain theanine administration; glycine and AMPA antagonists","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct brain dosing bypasses oral absorption and blood-brain-barrier transport. Antagonist sensitivity identifies pathway involvement, not necessarily direct theanine binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Conscious rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The glycine-receptor pathway participated in the dopamine response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p18196445] Theanine, gamma-glutamylethylamide, a unique amino acid in tea leaves, modulates neurotransmitter concentrations in the brain striatum interstitium in conscious rats. (2009). https://pubmed.ncbi.nlm.nih.gov/18196445/ DOI: 10.1007/s00726-007-0020-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Striatal extracellular neurotransmitters","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6a88a42c-3a6b-5eaf-ab0a-072982b71275","evidence_kind":"source_excerpt","locator":"Lines 380-391","start_line":380,"end_line":391,"excerpt":"### theanine-glycine-dopamine\nStrychnine reduced the theanine-induced dopamine response in rat striatum.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The glycine-receptor pathway participated in the dopamine response.\norganism: Conscious rats\ntissue_or_cell_type: Striatal extracellular neurotransmitters\nexperimental_model: In-vivo striatal microdialysis with receptor antagonists\nlimitations: Direct brain dosing bypasses oral absorption and blood-brain-barrier transport. Antagonist sensitivity identifies pathway involvement, not necessarily direct theanine binding.\nexposure: Local brain theanine administration; glycine and AMPA antagonists\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/18196445.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"59fdaf94d59c2f4b2981c29cd2981c043351aae1299a59a19e29979076957839\", \"start_char\": 0, \"end_char\": 1118, \"text_sha256\": \"59fdaf94d59c2f4b2981c29cd2981c043351aae1299a59a19e29979076957839\"}\n[theanine-p18196445] Theanine, gamma-glutamylethylamide, a unique amino acid in tea leaves, modulates neurotransmitter concentrations in the brain striatum interstitium in conscious rats. (2009). https://pubmed.ncbi.nlm.nih.gov/18196445/ DOI: 10.1007/s00726-007-0020-7","model_system":"In-vivo striatal microdialysis with receptor antagonists","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p18196445] Theanine, gamma-glutamylethylamide, a unique amino acid in tea leaves, modulates neurotransmitter concentrations in the brain striatum interstitium in conscious rats. (2009). https://pubmed.ncbi.nlm.nih.gov/18196445/ DOI: 10.1007/s00726-007-0020-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2bf3fc8-fc9a-5d50-937a-559a76f8be7d","stable_key":"import-8f12ba85-1a92-5337-a9c0-9cee9b3be90d","title":"L-Theanine: metabolism, neural signaling, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c78f2a1961da546cd8d3ae9131a16613aed0d1032904ca19175b0641647142c1","revision_id":"4ec64995-1e5b-5279-9102-f203cb2e29e5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}