{"id":"9cf06d9d-984b-59aa-9a15-ae71c4f0076e","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:slc41a1-sodium-independent-efflux","predicate":"supports","statement":"Mouse SLC41A1 expressed in HEK293 cells supported Mg extrusion without extracellular sodium in the Arjona isotope assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aefd4979-8df6-5abf-9a06-a6199be14668","mechanism_event_label":"A different experiment found magnesium export that did not require sodium.","subject":{"id":"d7454aa7-5fbe-57b8-b02b-25fff3d6b9bd","slug":"slc41a1","display_name":"SLC41A1","entity_type_key":"protein"},"object":{"id":"06a50d40-b240-5b4e-ba22-c5d2d1f138b7","slug":"cellular-magnesium-efflux","display_name":"Cellular magnesium efflux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"aefd4979-8df6-5abf-9a06-a6199be14668","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:slc41a1-sodium-independent-efflux-event","event_type":"biochemical_relationship","label":"A different experiment found magnesium export that did not require sodium.","description":"Mouse SLC41A1 expressed in HEK293 cells supported Mg extrusion without extracellular sodium in the Arjona isotope assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"exported-ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"tested-coupling-ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d7454aa7-5fbe-57b8-b02b-25fff3d6b9bd","slug":"slc41a1","display_name":"SLC41A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"06a50d40-b240-5b4e-ba22-c5d2d1f138b7","slug":"cellular-magnesium-efflux","display_name":"Cellular magnesium efflux","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"This assay challenges obligatory sodium dependence of SLC41A1-associated magnesium extrusion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence-system","value_text":"Mouse SLC41A1 construct; stable-isotope 25Mg measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse SLC41A1 construct; stable-isotope 25Mg measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different ortholog, loading and readout from the sodium-dependent report; physiological coupling remains unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein; human-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A different experiment found magnesium export that did not require sodium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[arjona-2019-slc41a1] SLC41A1 is essential for magnesium homeostasis in vivo (2019). https://pubmed.ncbi.nlm.nih.gov/30417250/ DOI: 10.1007/s00424-018-2234-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_direction","value_text":"Mg2+ from cytosol toward extracellular solution.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"caf6b8d7-7983-557b-b27d-eb25205fb5a3","evidence_kind":"source_excerpt","locator":"Lines 1180-1193","start_line":1180,"end_line":1193,"excerpt":"### slc41a1-sodium-independent-efflux\nMouse SLC41A1 expressed in HEK293 cells supported Mg extrusion without extracellular sodium in the Arjona isotope assay.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A different experiment found magnesium export that did not require sodium.\norganism: Mouse protein; human-derived cells\ntissue_or_cell_type: HEK293 cells\nexperimental_model: Mouse SLC41A1 construct; stable-isotope 25Mg measurements\nlimitations: Different ortholog, loading and readout from the sodium-dependent report; physiological coupling remains unresolved.\ncross_nutrient: This assay challenges obligatory sodium dependence of SLC41A1-associated magnesium extrusion.\ntransport_direction: Mg2+ from cytosol toward extracellular solution.\nevidence-system: Mouse SLC41A1 construct; stable-isotope 25Mg measurements\ntissue: HEK293 cells\n[arjona-2019-slc41a1] SLC41A1 is essential for magnesium homeostasis in vivo (2019). https://pubmed.ncbi.nlm.nih.gov/30417250/ DOI: 10.1007/s00424-018-2234-9","model_system":"Mouse SLC41A1 construct; stable-isotope 25Mg measurements","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [arjona-2019-slc41a1] SLC41A1 is essential for magnesium homeostasis in vivo (2019). https://pubmed.ncbi.nlm.nih.gov/30417250/ DOI: 10.1007/s00424-018-2234-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[{"id":"da79f532-7c45-508f-b26e-7101747c686b","title":"Is SLC41A1-associated magnesium efflux obligatorily sodium dependent?","kind":"contradiction","status":"open","why":"Human-SLC41A1 fluorescence experiments found strong Na dependence; mouse-SLC41A1 isotope experiments found efflux without Na.","resolution":"Unresolved across these protocols. Ortholog, expression, loading conditions and free-versus-isotope Mg readout differ. Keep both assay-scoped claims; do not encode obligatory exchange stoichiometry.","created_at":"2026-09-17 08:30:08","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/da79f532-7c45-508f-b26e-7101747c686b","sides":[{"conflict_id":"da79f532-7c45-508f-b26e-7101747c686b","ordinal":0,"label":"Kolisek: sodium-dependent efflux","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","start_line":1165,"end_line":1178,"quote":"### slc41a1-sodium-dependent-efflux\nHuman SLC41A1-associated Mg efflux in HEK293 cells fell sharply when extracellular sodium was replaced in the Kolisek assay.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This experiment supported sodium-coupled export of magnesium.\norganism: Human protein and human-derived cells\ntissue_or_cell_type: HEK293 cells\nexperimental_model: Human construct overexpression; mag-fura-2; extracellular Na replacement\nlimitations: Indirect free-ion assay and overexpression; conflicts with sodium-independent isotope flux in a different construct/protocol.\ncross_nutrient: Sodium availability affected measured magnesium export in this assay; coupling is contested.\ntransport_direction: Mg2+ from cytosol toward extracellular solution.\nevidence-system: Human construct overexpression; mag-fura-2; extracellular Na replacement\ntissue: HEK293 cells\n[kolisek-2012-slc41a1] Human gene SLC41A1 encodes for the Na+/Mg2+ exchanger (2012). https://pubmed.ncbi.nlm.nih.gov/22031603/ DOI: 10.1152/ajpcell.00289.2011","source_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","source_title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","claim_ids":["bd03a0a2-efa9-5dcf-af07-0286573b6bae"]},{"conflict_id":"da79f532-7c45-508f-b26e-7101747c686b","ordinal":1,"label":"Arjona: sodium-independent efflux","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","start_line":1180,"end_line":1193,"quote":"### slc41a1-sodium-independent-efflux\nMouse SLC41A1 expressed in HEK293 cells supported Mg extrusion without extracellular sodium in the Arjona isotope assay.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A different experiment found magnesium export that did not require sodium.\norganism: Mouse protein; human-derived cells\ntissue_or_cell_type: HEK293 cells\nexperimental_model: Mouse SLC41A1 construct; stable-isotope 25Mg measurements\nlimitations: Different ortholog, loading and readout from the sodium-dependent report; physiological coupling remains unresolved.\ncross_nutrient: This assay challenges obligatory sodium dependence of SLC41A1-associated magnesium extrusion.\ntransport_direction: Mg2+ from cytosol toward extracellular solution.\nevidence-system: Mouse SLC41A1 construct; stable-isotope 25Mg measurements\ntissue: HEK293 cells\n[arjona-2019-slc41a1] SLC41A1 is essential for magnesium homeostasis in vivo (2019). https://pubmed.ncbi.nlm.nih.gov/30417250/ DOI: 10.1007/s00424-018-2234-9","source_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","source_title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","claim_ids":["9cf06d9d-984b-59aa-9a15-ae71c4f0076e"]}]}],"corrections":[],"research":null}