{"id":"9c71d7de-92f3-5bdf-8c27-e1c82bd2ac7e","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-selk-dhhc6","predicate":"interacts_with","statement":"SELENOK and DHHC6 interacted at the ER membrane through SH3-related binding interactions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9786629a-c79b-538c-a418-677486da1f8c","mechanism_event_label":"Two separately identifiable proteins cooperate in maintaining the receptor.","subject":{"id":"83ea17c8-4611-596b-9be8-1a0df1c9b42e","slug":"selenok","display_name":"SELENOK","entity_type_key":"protein"},"object":{"id":"a2fd4b35-5483-5e7c-9604-d792fa67d402","slug":"zdhhc6","display_name":"ZDHHC6","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"9786629a-c79b-538c-a418-677486da1f8c","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-selk-dhhc6-event","event_type":"biochemical_relationship","label":"Two separately identifiable proteins cooperate in maintaining the receptor.","description":"SELENOK and DHHC6 interacted at the ER membrane through SH3-related binding interactions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83ea17c8-4611-596b-9be8-1a0df1c9b42e","slug":"selenok","display_name":"SELENOK","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a2fd4b35-5483-5e7c-9604-d792fa67d402","slug":"zdhhc6","display_name":"ZDHHC6","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/25368151.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\", \"start_char\": 0, \"end_char\": 1873, \"text_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Selk deletion, low-selenium media, DHHC6 knockdown and IP3R cysteine mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selenium limitation and genetic deletion are distinct. This does not establish that inositol supplements repair selenium-dependent receptor failure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mouse immune cells and mammalian cell models","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Two separately identifiable proteins cooperate in maintaining the receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. (2014). https://pubmed.ncbi.nlm.nih.gov/25368151/ DOI: 10.1073/pnas.1417176111","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"ER-associated IP3 receptor machinery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ca7262ae-0cca-5e7a-bc4b-ceecdce832f4","evidence_kind":"source_excerpt","locator":"Lines 1094-1105","start_line":1094,"end_line":1105,"excerpt":"### ino-selk-dhhc6\nSELENOK and DHHC6 interacted at the ER membrane through SH3-related binding interactions.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Two separately identifiable proteins cooperate in maintaining the receptor.\norganism: Mouse immune cells and mammalian cell models\ntissue_or_cell_type: ER-associated IP3 receptor machinery\nexperimental_model: Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays\nlimitations: Selenium limitation and genetic deletion are distinct. This does not establish that inositol supplements repair selenium-dependent receptor failure.\nexposure: Selk deletion, low-selenium media, DHHC6 knockdown and IP3R cysteine mutants\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/25368151.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\", \"start_char\": 0, \"end_char\": 1873, \"text_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\"}\n[ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. (2014). https://pubmed.ncbi.nlm.nih.gov/25368151/ DOI: 10.1073/pnas.1417176111","model_system":"Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. (2014). https://pubmed.ncbi.nlm.nih.gov/25368151/ DOI: 10.1073/pnas.1417176111","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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