{"id":"9c5df194-e8fe-5b26-85e9-0af2c511dc74","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-methylation","predicate":"methylates_to","statement":"Recombinant human CARNMT1 methylated carnosine to anserine; the reaction uses S-adenosylmethionine as methyl donor.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0d7266e1-2027-53d2-a922-c17487827177","mechanism_event_label":"A methyl group turns carnosine into a distinct peptide.","subject":{"id":"02c057af-ec05-545c-8bcb-73ef7b9b3401","slug":"human-carnmt1","display_name":"Human carnosine N-methyltransferase / CARNMT1","entity_type_key":"protein"},"object":{"id":"7fc34c5e-38d8-5b38-b3dd-f71c5d8dad47","slug":"anserine","display_name":"Anserine / beta-alanyl-N-pi-methyl-L-histidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0d7266e1-2027-53d2-a922-c17487827177","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-methylation-event","event_type":"observed_relationship","label":"A methyl group turns carnosine into a distinct peptide.","description":"Recombinant human CARNMT1 methylated carnosine to anserine; the reaction uses S-adenosylmethionine as methyl donor.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"02c057af-ec05-545c-8bcb-73ef7b9b3401","slug":"human-carnmt1","display_name":"Human carnosine N-methyltransferase / CARNMT1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7fc34c5e-38d8-5b38-b3dd-f71c5d8dad47","slug":"anserine","display_name":"Anserine / beta-alanyl-N-pi-methyl-L-histidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e84829b1-0607-558e-8add-aaa3af4747e7","slug":"s-adenosylhomocysteine","display_name":"S-Adenosyl-L-homocysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract plus Reactome reaction R-HSA-8876789","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human enzyme; reaction identity also curated in Reactome R-HSA-8876789.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish methyl-donor depletion or benefit from folate/B12 supplements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A methyl group turns carnosine into a distinct peptide.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"UPF0586 Protein C9orf41 Homolog Is Anserine-producing Methyltransferase. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26001783/ · DOI 10.1074/jbc.M115.640037","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7b38c8dd-fdba-5b97-a741-080851d92f92","evidence_kind":"source_excerpt","locator":"Lines 148-154","start_line":148,"end_line":154,"excerpt":"## carnosine-methylation\nA methyl group turns carnosine into a distinct peptide.\nRecombinant human CARNMT1 methylated carnosine to anserine; the reaction uses S-adenosylmethionine as methyl donor.\nModel: Purified human enzyme; reaction identity also curated in Reactome R-HSA-8876789.\nLimitations: Does not establish methyl-donor depletion or benefit from folate/B12 supplements.\nEvidence access: Primary abstract plus Reactome reaction R-HSA-8876789\nUPF0586 Protein C9orf41 Homolog Is Anserine-producing Methyltransferase. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26001783/ · DOI 10.1074/jbc.M115.640037","model_system":"Purified human enzyme; reaction identity also curated in Reactome R-HSA-8876789.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"469cbc38203db56bd031f9d8a2dd2bfa387713950ca30199511cd137e927aa7a","revision_id":"cc409a15-8b04-50ee-a7ff-d1bf32f686bb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}