{"id":"9c2a4486-1638-57c9-841a-f0074b3c9b93","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-lrat-phosphatidylcholine-donor","predicate":"donates-acyl-for","statement":"Human and rat liver LRAT activity transferred the sn-1 acyl group of phosphatidylcholine to CRBP-bound retinol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5d6defb4-e055-56c2-aee0-f4527af344b0","mechanism_event_label":"A membrane choline phospholipid supplies the fatty-acid group used to store vitamin A.","subject":{"id":"2f77ef6d-3ecd-5a80-9ca3-3cbea403d9fc","slug":"phosphatidylcholine","display_name":"Phosphatidylcholine","entity_type_key":"lipid"},"object":{"id":"37c06fa3-6d51-5e4f-8ec5-57869fd5fc2c","slug":"retinyl-ester-formation","display_name":"Retinyl ester formation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5d6defb4-e055-56c2-aee0-f4527af344b0","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-lrat-phosphatidylcholine-donor-event","event_type":"biochemical_relationship","label":"A membrane choline phospholipid supplies the fatty-acid group used to store vitamin A.","description":"Human and rat liver LRAT activity transferred the sn-1 acyl group of phosphatidylcholine to CRBP-bound retinol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"acyl-acceptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f7ce5851-df6d-54ed-b467-482480d26ff9","slug":"lrat","display_name":"Lecithin:retinol acyltransferase / LRAT","entity_type_key":"protein"},"role":"enzyme-activity","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e7f2697b-3ad8-5f52-849b-48cc0f1e9019","slug":"rbp1","display_name":"Cellular retinol-binding protein 1 / RBP1","entity_type_key":"protein"},"role":"retinol-carrier","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2f77ef6d-3ecd-5a80-9ca3-3cbea403d9fc","slug":"phosphatidylcholine","display_name":"Phosphatidylcholine","entity_type_key":"lipid"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"37c06fa3-6d51-5e4f-8ec5-57869fd5fc2c","slug":"retinyl-ester-formation","display_name":"Retinyl ester formation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Phosphatidylcholine-dependent retinol storage links retinoid metabolism to choline-containing phospholipid availability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human and rat liver microsomes supplied phosphatidylcholine and CRBP-bound retinol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Exogenous phosphatidylcholine plus CRBP-bound retinol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical donor requirement; dietary choline deficiency was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens and Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"outcome","value_text":"Human and rat liver LRAT activity transferred the sn-1 acyl group of phosphatidylcholine to CRBP-bound retinol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A membrane choline phospholipid supplies the fatty-acid group used to store vitamin A.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-macdonald-1988] A lecithin:retinol acyltransferase activity in human and rat liver (1988). https://pubmed.ncbi.nlm.nih.gov/3178828/ DOI: 10.1016/S0006-291X(88)80818-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver microsomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8a714c11-19ce-5def-aeb2-bd1656c168ff","evidence_kind":"source_excerpt","locator":"Lines 317-330","start_line":317,"end_line":330,"excerpt":"### va-lrat-phosphatidylcholine-donor\nHuman and rat liver LRAT activity transferred the sn-1 acyl group of phosphatidylcholine to CRBP-bound retinol.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A membrane choline phospholipid supplies the fatty-acid group used to store vitamin A.\norganism: Homo sapiens and Rattus norvegicus\ntissue_or_cell_type: Liver microsomes\nexperimental_model: Human and rat liver microsomes supplied phosphatidylcholine and CRBP-bound retinol.\nlimitations: Biochemical donor requirement; dietary choline deficiency was not tested.\nexposure: Exogenous phosphatidylcholine plus CRBP-bound retinol.\noutcome: Human and rat liver LRAT activity transferred the sn-1 acyl group of phosphatidylcholine to CRBP-bound retinol.\nevidence_location: Abstract\ncross_nutrient: Phosphatidylcholine-dependent retinol storage links retinoid metabolism to choline-containing phospholipid availability.\n[va-macdonald-1988] A lecithin:retinol acyltransferase activity in human and rat liver (1988). https://pubmed.ncbi.nlm.nih.gov/3178828/ DOI: 10.1016/S0006-291X(88)80818-0","model_system":"Human and rat liver microsomes supplied phosphatidylcholine and CRBP-bound retinol.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-macdonald-1988] A lecithin:retinol acyltransferase activity in human and rat liver (1988). https://pubmed.ncbi.nlm.nih.gov/3178828/ DOI: 10.1016/S0006-291X(88)80818-0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}