{"id":"9bec9c2f-c099-5f84-9176-4944ae022af1","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lox-hydroxylysine-oxidation","predicate":"oxidatively_deaminates","statement":"Lysyl-oxidase activity can convert collagen hydroxylysine side chains to hydroxyallysine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d8a7bf21-779d-55a1-a98c-a314a3357ad0","mechanism_event_label":"Hydroxylated lysines provide a different aldehyde starting point for cross-links.","subject":{"id":"815f6321-8845-5ab5-bbd2-90872ac2d032","slug":"lysyl-oxidase-family","display_name":"Lysyl oxidase enzyme family","entity_type_key":"protein_family"},"object":{"id":"7c465ab2-233e-5401-9706-3492e9ea8f45","slug":"collagen-hydroxylysine-residues","display_name":"Collagen-bound 5-hydroxylysine residues","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"d8a7bf21-779d-55a1-a98c-a314a3357ad0","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lox-hydroxylysine-oxidation-event","event_type":"biochemical_relationship","label":"Hydroxylated lysines provide a different aldehyde starting point for cross-links.","description":"Lysyl-oxidase activity can convert collagen hydroxylysine side chains to hydroxyallysine.","status":"provisional","compartment":{"slug":"extracellular-space","display_name":"Extracellular space"},"participants":[{"entity":{"id":"815f6321-8845-5ab5-bbd2-90872ac2d032","slug":"lysyl-oxidase-family","display_name":"Lysyl oxidase enzyme family","entity_type_key":"protein_family"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7c465ab2-233e-5401-9706-3492e9ea8f45","slug":"collagen-hydroxylysine-residues","display_name":"Collagen-bound 5-hydroxylysine residues","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bdd7dfe3-b5f2-55e0-a28d-c67a33c47fb3","slug":"loxl2","display_name":"LOXL2","entity_type_key":"protein"},"role":"experimentally_tested_family_member","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1260166d-0e74-5794-9853-3518f9fde655","slug":"collagen-hydroxyallysine","display_name":"Collagen peptidyl hydroxyallysine residues","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"1374ec24-1a5b-552b-982f-51f9082f4187","slug":"ammonia","display_name":"Ammonia","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"LOXL2 assay development with extracellular matrix; family-level reaction context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a reaction-class record; not every hydroxylysine site is an accessible LOX substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mammalian cells/tissues and recombinant LOXL2; see study methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Hydroxylated lysines provide a different aldehyde starting point for cross-links.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lox-assay-2021] An in situ activity assay for lysyl oxidases (2021). https://pubmed.ncbi.nlm.nih.gov/34226627/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b278632d-9b14-5244-9534-d42c2a2ae48f","evidence_kind":"source_excerpt","locator":"Lines 477-485","start_line":477,"end_line":485,"excerpt":"### lox-hydroxylysine-oxidation\nLysyl-oxidase activity can convert collagen hydroxylysine side chains to hydroxyallysine.\nPlain language: Hydroxylated lysines provide a different aldehyde starting point for cross-links.\nCondition category: normal\norganism: Mammalian cells/tissues and recombinant LOXL2; see study methods\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: LOXL2 assay development with extracellular matrix; family-level reaction context.\nlimitations: This is a reaction-class record; not every hydroxylysine site is an accessible LOX substrate.\n[lox-assay-2021] An in situ activity assay for lysyl oxidases (2021). https://pubmed.ncbi.nlm.nih.gov/34226627/","model_system":"LOXL2 assay development with extracellular matrix; family-level reaction context.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [lox-assay-2021] An in situ activity assay for lysyl oxidases (2021). https://pubmed.ncbi.nlm.nih.gov/34226627/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}