{"id":"9be949be-886f-5b05-bcef-2d1def8eb590","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-aanat","predicate":"reduces_tested","statement":"The pineal melatonin reduction was partly attributed to lower norepinephrine-stimulated serotonin N-acetyltransferase activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"45ab6ba8-0a7e-50d0-ab99-0297569a35eb","mechanism_event_label":"A step in converting serotonin toward melatonin was less active.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"0d803df7-aaaa-5c3a-b2ff-969f4ac7500c","slug":"rat-aanat","display_name":"Rat aralkylamine N-acetyltransferase / Aanat","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"45ab6ba8-0a7e-50d0-ab99-0297569a35eb","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-aanat-event","event_type":"observed_relationship","label":"A step in converting serotonin toward melatonin was less active.","description":"The pineal melatonin reduction was partly attributed to lower norepinephrine-stimulated serotonin N-acetyltransferase activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0d803df7-aaaa-5c3a-b2ff-969f4ac7500c","slug":"rat-aanat","display_name":"Rat aralkylamine N-acetyltransferase / Aanat","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pineal preparation enzyme-activity measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial mediation; does not establish direct binding of D-aspartate to AANAT.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A step in converting serotonin toward melatonin was less active.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"D-aspartate modulates melatonin synthesis in rat pinealocytes. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9682837/ · DOI 10.1016/s0304-3940(98)00414-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"66e83a97-f949-540e-99b9-6dcfc6c0d93e","evidence_kind":"source_excerpt","locator":"Lines 184-190","start_line":184,"end_line":190,"excerpt":"## d-aspartate-pineal-aanat\nA step in converting serotonin toward melatonin was less active.\nThe pineal melatonin reduction was partly attributed to lower norepinephrine-stimulated serotonin N-acetyltransferase activity.\nModel: Rat pineal preparation enzyme-activity measurements.\nLimitations: Partial mediation; does not establish direct binding of D-aspartate to AANAT.\nEvidence access: Primary abstract\nD-aspartate modulates melatonin synthesis in rat pinealocytes. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9682837/ · DOI 10.1016/s0304-3940(98)00414-5","model_system":"Rat pineal preparation enzyme-activity measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0a86a1008edb8a69dcc79409e76e3fd5ce59b0747be8cae8f21e43f871e76248","revision_id":"a1de4b66-263d-5452-8ed8-df853afba030","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}