{"id":"9bb94e7e-5161-5424-8e0e-dc90d48689ad","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-hnrnpa2-binding","predicate":"reported_relationship","statement":"Apigenin bound the glycine-rich C-terminal region of hnRNPA2 and inhibited its homodimerization in the reported biochemical experiments.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"182ba75c-ba2f-501d-9e12-686287c1289b","mechanism_event_label":"Apigenin bound the glycine-rich C-terminal region of hnRNPA2 and inhibited its homodimerization in the reported biochemical experiments.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"852a7b7e-532b-51e6-bb1a-de63dac60035","slug":"human-hnrnpa2b1","display_name":"Human heterogeneous nuclear ribonucleoprotein A2/B1 / HNRNPA2B1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"182ba75c-ba2f-501d-9e12-686287c1289b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-hnrnpa2-binding-event","event_type":"biochemical_relationship","label":"Apigenin bound the glycine-rich C-terminal region of hnRNPA2 and inhibited its homodimerization in the reported biochemical experiments.","description":"### api-hnrnpa2-binding\n\nApigenin bound the glycine-rich C-terminal region of hnRNPA2 and inhibited its homodimerization in the reported biochemical experiments.\nThe screen nominated 160 candidate proteins; only selected targets received functional follow-up. Do not label all 160 validated causal targets or dietary target engagement.\nModel and exposure: Human protein fragments, affinity pull-down and follow-up assays.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets. | 2013 | https://pubmed.ncbi.nlm.nih.gov/23697369/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3683737/ | https://doi.org/10.1073/pnas.1303726110 | Access: Primary indexed abstract plus PMC full-text Results retrieved through web; no claim that every screening hit is functionally validated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"852a7b7e-532b-51e6-bb1a-de63dac60035","slug":"human-hnrnpa2b1","display_name":"Human heterogeneous nuclear ribonucleoprotein A2/B1 / HNRNPA2B1","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract plus PMC full-text Results retrieved through web; no claim that every screening hit is functionally validated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human protein fragments, affinity pull-down and follow-up assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin bound the glycine-rich C-terminal region of hnRNPA2 and inhibited its homodimerization in the reported biochemical experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets. | https://pubmed.ncbi.nlm.nih.gov/23697369/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3683737/ | https://doi.org/10.1073/pnas.1303726110","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-hnrnpa2-binding; The screen nominated 160 candidate proteins; only selected targets received functional follow-up. Do not label all 160 validated causal targets or dietary target engagement.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"af439c5a-f87e-5a88-9817-037da87e2cd9","evidence_kind":"source_excerpt","locator":"Lines 644-650","start_line":644,"end_line":650,"excerpt":"### api-hnrnpa2-binding\n\nApigenin bound the glycine-rich C-terminal region of hnRNPA2 and inhibited its homodimerization in the reported biochemical experiments.\nThe screen nominated 160 candidate proteins; only selected targets received functional follow-up. Do not label all 160 validated causal targets or dietary target engagement.\nModel and exposure: Human protein fragments, affinity pull-down and follow-up assays.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets. | 2013 | https://pubmed.ncbi.nlm.nih.gov/23697369/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3683737/ | https://doi.org/10.1073/pnas.1303726110 | Access: Primary indexed abstract plus PMC full-text Results retrieved through web; no claim that every screening hit is functionally validated.","model_system":"Human protein fragments, affinity pull-down and follow-up assays.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}