{"id":"9ba7df88-d128-5d54-9bdc-2a5f3c7b2dac","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-nfs1-mocs3-transfer","predicate":"transfers_sulfur_to","statement":"Purified NFS1 interacted specifically with MOCS3-RLD and supported sulfur transfer to it.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e8c1b57c-7000-5e01-b3ba-89e9169f617c","mechanism_event_label":"The sulfur handoff uses identifiable proteins, not a free-floating mineral pool.","subject":{"id":"6fec6f2a-0e68-575d-9307-e509e69d4ae1","slug":"nfs1-persulfide","display_name":"Human NFS1 sulfur-loaded persulfide state","entity_type_key":"protein_state"},"object":{"id":"c9f482f8-899b-5a59-9745-05a8709d91a2","slug":"mocs3","display_name":"Human adenylyltransferase and sulfurtransferase / MOCS3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e8c1b57c-7000-5e01-b3ba-89e9169f617c","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-nfs1-mocs3-transfer-event","event_type":"biochemical_relationship","label":"The sulfur handoff uses identifiable proteins, not a free-floating mineral pool.","description":"Purified NFS1 interacted specifically with MOCS3-RLD and supported sulfur transfer to it.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"465f4f2d-1739-5e2b-b6be-fbf625620555","slug":"mocs3-persulfide","display_name":"Human MOCS3 sulfur-loaded persulfide state","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6fec6f2a-0e68-575d-9307-e509e69d4ae1","slug":"nfs1-persulfide","display_name":"Human NFS1 sulfur-loaded persulfide state","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c9f482f8-899b-5a59-9745-05a8709d91a2","slug":"mocs3","display_name":"Human adenylyltransferase and sulfurtransferase / MOCS3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/18650437.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8180bd148722e8980ef5acbd4ae4c29a0d9d13da56bbccb91165a5eed4ab6f50\", \"start_char\": 0, \"end_char\": 1547, \"text_sha256\": \"8180bd148722e8980ef5acbd4ae4c29a0d9d13da56bbccb91165a5eed4ab6f50\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human NFS1/ISD11 and MOCS3 rhodanese-domain interaction and sulfur-transfer assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"L-cysteine sulfur-donor assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Truncated and heterologously expressed proteins; cell localization was investigated independently in 2013.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human proteins expressed in Escherichia coli","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The sulfur handoff uses identifiable proteins, not a free-floating mineral pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p18650437] A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis. (2008). https://pubmed.ncbi.nlm.nih.gov/18650437/ DOI: 10.1074/jbc.m804064200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified proteins","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e518f24f-c29f-5777-90cf-3eb176305007","evidence_kind":"source_excerpt","locator":"Lines 365-376","start_line":365,"end_line":376,"excerpt":"### mo-nfs1-mocs3-transfer\nPurified NFS1 interacted specifically with MOCS3-RLD and supported sulfur transfer to it.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The sulfur handoff uses identifiable proteins, not a free-floating mineral pool.\norganism: Human proteins expressed in Escherichia coli\ntissue_or_cell_type: Purified proteins\nexperimental_model: Purified human NFS1/ISD11 and MOCS3 rhodanese-domain interaction and sulfur-transfer assays\nlimitations: Truncated and heterologously expressed proteins; cell localization was investigated independently in 2013.\nexposure: L-cysteine sulfur-donor assays\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/18650437.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8180bd148722e8980ef5acbd4ae4c29a0d9d13da56bbccb91165a5eed4ab6f50\", \"start_char\": 0, \"end_char\": 1547, \"text_sha256\": \"8180bd148722e8980ef5acbd4ae4c29a0d9d13da56bbccb91165a5eed4ab6f50\"}\n[mo-p18650437] A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis. (2008). https://pubmed.ncbi.nlm.nih.gov/18650437/ DOI: 10.1074/jbc.m804064200","model_system":"Purified human NFS1/ISD11 and MOCS3 rhodanese-domain interaction and sulfur-transfer assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p18650437] A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis. 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