{"id":"9b9f9161-8d06-55a8-98ce-53441909d482","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nos1-inhibition","predicate":"competitively_inhibits","statement":"Agmatine competitively inhibited the brain-derived NOS I preparation with approximate Ki 660 micromolar and was not an NO precursor.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c2b442d4-f7ac-52d1-ad34-7425859a8fc1","mechanism_event_label":"An arginine-related compound can inhibit the enzyme rather than supply its product.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"2781b74d-4b05-590a-a13b-8fcc64891f85","slug":"nos1-1996-agmatine-assay","display_name":"Brain-derived NOS I in the 1996 agmatine assay, species unverified","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c2b442d4-f7ac-52d1-ad34-7425859a8fc1","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nos1-inhibition-event","event_type":"observed_relationship","label":"An arginine-related compound can inhibit the enzyme rather than supply its product.","description":"Agmatine competitively inhibited the brain-derived NOS I preparation with approximate Ki 660 micromolar and was not an NO precursor.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2781b74d-4b05-590a-a13b-8fcc64891f85","slug":"nos1-1996-agmatine-assay","display_name":"Brain-derived NOS I in the 1996 agmatine assay, species unverified","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f53c7bfb-3b91-56fe-bebd-28623cfe13a0","slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"NOS activity measured by nitrite or citrulline production; species not specified in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isolated-preparation potency does not quantify inhibition after an oral sulfate dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An arginine-related compound can inhibit the enzyme rather than supply its product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8645212/ · DOI 10.1042/bj3160247","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d074090d-dbdf-5788-864a-4df8568b931d","evidence_kind":"source_excerpt","locator":"Lines 220-226","start_line":220,"end_line":226,"excerpt":"## agmatine-sulfate-nos1-inhibition\nAn arginine-related compound can inhibit the enzyme rather than supply its product.\nAgmatine competitively inhibited the brain-derived NOS I preparation with approximate Ki 660 micromolar and was not an NO precursor.\nModel: NOS activity measured by nitrite or citrulline production; species not specified in accessed abstract.\nLimitations: Isolated-preparation potency does not quantify inhibition after an oral sulfate dose.\nEvidence access: Primary abstract\nInhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8645212/ · DOI 10.1042/bj3160247","model_system":"NOS activity measured by nitrite or citrulline production; species not specified in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}