{"id":"9b819324-4157-510f-819c-18f82def6180","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-thiamine-inhibits-pyridoxine-uptake","predicate":"inhibits","statement":"Thiamine inhibited SLC19A2- and SLC19A3-mediated pyridoxine uptake in acidic-pH transfectant assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fd2f634c-65cb-5be7-996f-0fefa7ebf074","mechanism_event_label":"B1 reduced uptake of a B6 form in these transporter experiments.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"ee31e9c6-9993-5fb0-807a-02933e28a1bc","slug":"cellular-pyridoxine-uptake","display_name":"Cellular pyridoxine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fd2f634c-65cb-5be7-996f-0fefa7ebf074","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-thiamine-inhibits-pyridoxine-uptake-event","event_type":"biochemical_relationship","label":"B1 reduced uptake of a B6 form in these transporter experiments.","description":"Thiamine inhibited SLC19A2- and SLC19A3-mediated pyridoxine uptake in acidic-pH transfectant assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"role":"uptake substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c9e677e1-db84-51e4-8a27-911614687d5f","slug":"slc19a2","display_name":"Human thiamine transporter 1 / SLC19A2","entity_type_key":"protein"},"role":"tested transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4eba176e-17c8-5515-920a-df5d48a40486","slug":"slc19a3","display_name":"Human thiamine transporter 2 / SLC19A3","entity_type_key":"protein"},"role":"tested transporter","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ee31e9c6-9993-5fb0-807a-02933e28a1bc","slug":"cellular-pyridoxine-uptake","display_name":"Cellular pyridoxine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Direct B1-to-pyridoxine transport inhibition is demonstrated in vitro, with pH and exposure limitations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence-scope","value_text":"Cultured epithelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_locator","value_text":"Figures 1-3, 6 and 9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_document\": \"artifacts/thiamine_transport_sources/yamashiro-2020-pyridoxine-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1718}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter constructs in MDCKII/HEK293 cells; 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topical membership is not evidence of a direct dietary effect.\nplain_language: B1 reduced uptake of a B6 form in these transporter experiments.\norganism: Human proteins; canine and human cell hosts\ntissue_or_cell_type: Cultured epithelial cells\nexperimental_model: Human transporter constructs in MDCKII/HEK293 cells; radiotracer uptake at pH 5.5.\nlimitations: Assay inhibition does not show clinical B6 deficiency from dietary thiamine.\ncross_nutrient: Direct B1-to-pyridoxine transport inhibition is demonstrated in vitro, with pH and exposure limitations.\nevidence_spans: [{\"source_document\": \"artifacts/thiamine_transport_sources/yamashiro-2020-pyridoxine-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1718}]\nevidence_locator: Figures 1-3, 6 and 9\nextracellular-pH: 5.5\nevidence-scope: Cultured epithelial cells\n[yamashiro-2020-pyridoxine] pH-dependent pyridoxine transport by SLC19A2 and SLC19A3: Implications for absorption in acidic microclimates (2020). https://pmc.ncbi.nlm.nih.gov/articles/PMC7863892/ DOI: 10.1074/jbc.RA120.013610","model_system":"Human transporter constructs in MDCKII/HEK293 cells; radiotracer uptake at pH 5.5.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [yamashiro-2020-pyridoxine] pH-dependent pyridoxine transport by SLC19A2 and SLC19A3: Implications for absorption in acidic microclimates (2020). https://pmc.ncbi.nlm.nih.gov/articles/PMC7863892/ DOI: 10.1074/jbc.RA120.013610","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}