{"id":"9af7cf72-584a-59b9-9d38-70408c7910e1","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-mouse-acp-isu-stability","predicate":"depletion_reduces","statement":"Ndufab1 siRNA in mouse C2C12 myoblasts reduced mitochondrial Nfs1, Isd11 and Iscu2 protein abundance.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"49a7f84b-973f-5f82-9ac0-a2997a5b205d","mechanism_event_label":"The iron–sulfur machinery also became less stable after carrier depletion in mouse muscle cells.","subject":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"object":{"id":"f5cef903-677c-5210-8a13-37f7626fd89f","slug":"mouse-nfs1","display_name":"Mouse cysteine desulfurase / Nfs1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"49a7f84b-973f-5f82-9ac0-a2997a5b205d","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-mouse-acp-isu-stability-event","event_type":"biochemical_relationship","label":"The iron–sulfur machinery also became less stable after carrier depletion in mouse muscle cells.","description":"Ndufab1 siRNA in mouse C2C12 myoblasts reduced mitochondrial Nfs1, Isd11 and Iscu2 protein abundance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83c5d0c8-ce4b-5234-bb03-5fc630a070f8","slug":"mouse-lyrm4","display_name":"Mouse ISD11 / Lyrm4","entity_type_key":"protein"},"role":"coaffected_component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"de714b3e-6d37-55e5-af8a-55a8269589d6","slug":"mouse-iscu","display_name":"Mouse iron-sulfur cluster scaffold / Iscu","entity_type_key":"protein"},"role":"coaffected_scaffold","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"normal_carrier_prosthetic_group","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f5cef903-677c-5210-8a13-37f7626fd89f","slug":"mouse-nfs1","display_name":"Mouse cysteine desulfurase / Nfs1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text line 117; Fig. 3C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pooled Ndufab1 siRNA versus scrambled control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Ndufab1-targeting siRNA; immunoblot of isolated mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The mammalian intact Nfs1–Isd11 complex could not be resolved by the authors with BN-PAGE; the direct mammalian result is subunit abundance. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The iron–sulfur machinery also became less stable after carrier depletion in mouse muscle cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"C2C12 myoblast mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"949c7c34-42d8-55b2-9fdc-de3dd6c04048","evidence_kind":"source_excerpt","locator":"Lines 921-933","start_line":921,"end_line":933,"excerpt":"### b5-met-mouse-acp-isu-stability\nNdufab1 siRNA in mouse C2C12 myoblasts reduced mitochondrial Nfs1, Isd11 and Iscu2 protein abundance.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The iron–sulfur machinery also became less stable after carrier depletion in mouse muscle cells.\norganism: Mus musculus\ntissue_or_cell_type: C2C12 myoblast mitochondria\nexperimental_model: Pooled Ndufab1 siRNA versus scrambled control\nlimitations: The mammalian intact Nfs1–Isd11 complex could not be resolved by the authors with BN-PAGE; the direct mammalian result is subunit abundance. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Ndufab1-targeting siRNA; immunoblot of isolated mitochondria.\ncross_nutrient: true\nevidence_location: Full text line 117; Fig. 3C\n[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","model_system":"Pooled Ndufab1 siRNA versus scrambled control","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}