{"id":"9ae71e5b-b18e-5dfd-a2a3-049b8c963e6d","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-mitochondrial-energy","predicate":"supports","statement":"Fars2 knockdown in neonatal rat ventricular myocytes lowered ATP and mitochondrial membrane potential, increased ROS, and reduced the NAD+/NADH ratio.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7be08bf6-92a3-5c9b-b099-d116c4ca9a81","mechanism_event_label":"The downstream effects include both energy production and redox balance.","subject":{"id":"4538bb52-bd32-57eb-986e-0a0fb4161848","slug":"rat-fars2","display_name":"Rat mitochondrial phenylalanyl-tRNA synthetase / Fars2","entity_type_key":"protein"},"object":{"id":"78f895d5-74bd-594a-8785-7d172b5e282e","slug":"rat-fars2-mitochondrial-energy","display_name":"Rat cardiomyocyte mitochondrial energy and redox state","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7be08bf6-92a3-5c9b-b099-d116c4ca9a81","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-mitochondrial-energy-event","event_type":"observed_relationship","label":"The downstream effects include both energy production and redox balance.","description":"Fars2 knockdown in neonatal rat ventricular myocytes lowered ATP and mitochondrial membrane potential, increased ROS, and reduced the NAD+/NADH ratio.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4538bb52-bd32-57eb-986e-0a0fb4161848","slug":"rat-fars2","display_name":"Rat mitochondrial phenylalanyl-tRNA synthetase / Fars2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"78f895d5-74bd-594a-8785-7d172b5e282e","slug":"rat-fars2-mitochondrial-energy","display_name":"Rat cardiomyocyte mitochondrial energy and redox state","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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