{"id":"9abc54fa-a010-5d3e-aeb7-f0ca73781c46","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-mouse-axl-osteoclast","predicate":"inhibition_reduces","statement":"Pharmacological inhibition of mouse-axl blocked osteoclast generation in the co-culture system.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"560281bb-0c92-57cb-8140-218295406b81","mechanism_event_label":"The receptor is a separate intervention point in this pathway.","subject":{"id":"be1e3ebb-90e7-55d4-a08d-bb24aa719720","slug":"mouse-axl","display_name":"Mouse Axl receptor tyrosine kinase","entity_type_key":"protein"},"object":{"id":"c2ed1022-1a1b-5ffe-b415-d37791b53f24","slug":"osteoclast-maturation","display_name":"Osteoclast maturation and multinucleation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"560281bb-0c92-57cb-8140-218295406b81","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-mouse-axl-osteoclast-event","event_type":"biochemical_relationship","label":"The receptor is a separate intervention point in this pathway.","description":"Pharmacological inhibition of mouse-axl blocked osteoclast generation in the co-culture system.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ee14083-161a-58f7-b06e-9515a84aabef","slug":"mouse-gas6","display_name":"Mouse growth arrest-specific protein 6 / Gas6","entity_type_key":"protein"},"role":"upstream_ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"be1e3ebb-90e7-55d4-a08d-bb24aa719720","slug":"mouse-axl","display_name":"Mouse Axl receptor tyrosine kinase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c2ed1022-1a1b-5ffe-b415-d37791b53f24","slug":"osteoclast-maturation","display_name":"Osteoclast maturation and multinucleation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/k2-research/42045170.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"590b559787319a57a3e2fae4164fc2f2a40761ce653bd426d34a2d76aff5d4cf\", \"start_char\": 0, \"end_char\": 1703, \"text_sha256\": \"590b559787319a57a3e2fae4164fc2f2a40761ce653bd426d34a2d76aff5d4cf\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Osteoblast-specific deletion, co-culture, receptor inhibitors and transgenic overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Osteoblast Ggcx deletion; carboxylated GAS6 and AXL/MERTK perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"2026 mouse study; increased bone mass is not proven improved human bone quality, and no oral MK-7 regimen was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"k2","display_name":"Vitamin K2 / menaquinone family","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Male mice and mouse osteoblast/osteoclast cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The receptor is a separate intervention point in this pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[k2-p42045170] Vitamin K-dependent carboxylation in osteoblasts regulates bone resorption through GAS6 in male mice. 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