{"id":"9a973d56-90c0-547c-b68c-92972aa14281","stable_key":"research:acidified-vesicles-enable-selenop-use","predicate":"supports","statement":"Vesicle acidification was required for efficient selenium utilization from SELENOP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"02227703-5e3c-59d8-a06f-2717dc4cd1e4","mechanism_event_label":"Cells processed SELENOP in acidified compartments.","subject":{"id":"ceebbad1-e8a8-5d14-a9d5-3df779d4620e","slug":"seleno-p-vesicle-acidification","display_name":"SELENOP-containing vesicle acidification","entity_type_key":"cellular_process"},"object":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"02227703-5e3c-59d8-a06f-2717dc4cd1e4","stable_key":"research:acidified-vesicles-enable-selenop-use","event_type":"processing-dependence","label":"Cells processed SELENOP in acidified compartments.","description":"Vesicle acidification was required for efficient selenium utilization from SELENOP.","status":"active","compartment":{"slug":"intracellular-vesicles","display_name":"Intracellular vesicles"},"participants":[{"entity":{"id":"ceebbad1-e8a8-5d14-a9d5-3df779d4620e","slug":"seleno-p-vesicle-acidification","display_name":"SELENOP-containing vesicle acidification","entity_type_key":"cellular_process"},"role":"required-process","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c7fbd153-6444-50b2-a8be-6936e352a11c","slug":"selenop","display_name":"SELENOP","entity_type_key":"protein"},"role":"selenium-source","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"role":"readout","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"Jurkat and RD","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Acidification inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chemical recovery steps are incompletely resolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ecf6ac0b-0777-5f80-ae59-6c84a54dad7f","evidence_kind":"curated_literature_summary","locator":"lines 558-568","start_line":558,"end_line":568,"excerpt":"## acidified-vesicles-enable-selenop-use\n\nCells processed SELENOP in acidified compartments.\n\nVesicle acidification was required for efficient selenium utilization from SELENOP.\n\nOrganism: human\nCell type: Jurkat and RD\nExperimental model: Acidification inhibition\nLimitations: Chemical recovery steps are incompletely resolved.\nPrimary reference: [An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase](https://pubmed.ncbi.nlm.nih.gov/37406814/)","model_system":"Acidification inhibition","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"selenium-transport","plain_language":"Cells processed SELENOP in acidified compartments.","evidence_scope":"direct_experimental","papers":[{"key":"mizuno-2023-lrp8-routing","title":"An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase","url":"https://pubmed.ncbi.nlm.nih.gov/37406814/","doi":"10.1016/j.jbc.2023.105009","year":2023,"model":"Human Jurkat and RD cells","summary":"Receptor variants and vesicle processing differ between cell lines; SCLY dependence is not universal."}]}}