{"id":"9a927ca7-d849-53fa-ab07-03ea53070a0d","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-claudin-rescue","predicate":"when_overexpressed_attenuates","statement":"Claudin-3 overexpression attenuated aspartame-associated permeability and ROS changes in Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"be63f258-e74e-5131-9e15-aa8bbfe5ea21","mechanism_event_label":"Restoring a barrier component altered downstream readouts.","subject":{"id":"2ccc9999-f1db-5fb7-b57c-513323c3e26e","slug":"cldn3","display_name":"Human claudin 3 / CLDN3","entity_type_key":"protein"},"object":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"be63f258-e74e-5131-9e15-aa8bbfe5ea21","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-claudin-rescue-event","event_type":"observed_relationship","label":"Restoring a barrier component altered downstream readouts.","description":"Claudin-3 overexpression attenuated aspartame-associated permeability and ROS changes in Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2ccc9999-f1db-5fb7-b57c-513323c3e26e","slug":"cldn3","display_name":"Human claudin 3 / CLDN3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"afffe733-49d5-55e9-8895-6e5e78d0f534","slug":"human-caco2-aspartame-ros","display_name":"Human Caco-2 ROS signal during aspartame exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell-line expression rescue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish that the same rescue is clinically achievable.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Restoring a barrier component altered downstream readouts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Artificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"81d4d92d-3641-59d1-ae71-b048d6d3c6ab","evidence_kind":"source_excerpt","locator":"Lines 234-240","start_line":234,"end_line":240,"excerpt":"## aspartame-claudin-rescue\nRestoring a barrier component altered downstream readouts.\nClaudin-3 overexpression attenuated aspartame-associated permeability and ROS changes in Caco-2 cells.\nModel: Human cell-line expression rescue.\nLimitations: Does not establish that the same rescue is clinically achievable.\nEvidence access: Primary full text\nArtificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","model_system":"Human cell-line expression rescue.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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