{"id":"9a892d39-b67d-57f9-a1b3-f9438c6a90a4","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyat-variant","predicate":"reduces_tested","statement":"The human GLYAT N156S/R199C variant had turnover about 9.8% of the common N156S reference haplotype in vitro despite greater apparent benzoyl-CoA affinity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f4ceb3c4-4b37-5dbb-ab80-b9779c0af92e","mechanism_event_label":"Tighter apparent binding did not mean faster detoxification.","subject":{"id":"fc94b496-c32b-553b-8228-0f6a7dfae18d","slug":"human-glyat-n156s-r199c","display_name":"Human GLYAT N156S/R199C haplotype","entity_type_key":"protein"},"object":{"id":"f1288417-59ea-5f8e-8112-bf5987d077a1","slug":"human-glyat-benzoyl-turnover","display_name":"Human GLYAT benzoyl-CoA turnover","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f4ceb3c4-4b37-5dbb-ab80-b9779c0af92e","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyat-variant-event","event_type":"observed_relationship","label":"Tighter apparent binding did not mean faster detoxification.","description":"The human GLYAT N156S/R199C variant had turnover about 9.8% of the common N156S reference haplotype in vitro despite greater apparent benzoyl-CoA affinity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fc94b496-c32b-553b-8228-0f6a7dfae18d","slug":"human-glyat-n156s-r199c","display_name":"Human GLYAT N156S/R199C haplotype","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f1288417-59ea-5f8e-8112-bf5987d077a1","slug":"human-glyat-benzoyl-turnover","display_name":"Human GLYAT benzoyl-CoA turnover","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3febb8ae-e5ee-51ef-912f-826f4e2e3ddd","slug":"glyat","display_name":"Human glycine N-acyltransferase / GLYAT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e3f62386-3053-5d49-a6d3-fe9e92a8df63","slug":"benzoyl-coa","display_name":"Benzoyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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unverified.","entity":null}],"evidence":[{"id":"63fc5af7-5a8e-5939-8acf-30042ff9c066","evidence_kind":"source_excerpt","locator":"Lines 282-288","start_line":282,"end_line":288,"excerpt":"## glycine-glyat-variant\nTighter apparent binding did not mean faster detoxification.\nThe human GLYAT N156S/R199C variant had turnover about 9.8% of the common N156S reference haplotype in vitro despite greater apparent benzoyl-CoA affinity.\nModel: Recombinant haplotype kinetics; not a patient treatment trial.\nLimitations: Potential clinical consequences remain untested; do not equate genotype with a known glycine-deficiency syndrome.\nEvidence access: Primary full text\nFunctional Characterisation of Three Glycine N-Acyltransferase Variants and the Effect on Glycine Conjugation to Benzoyl-CoA. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33803916/ · DOI 10.3390/ijms22063129","model_system":"Recombinant haplotype kinetics; not a patient treatment trial.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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