{"id":"9a84b582-5aa9-533b-a6d2-694b640683fb","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-native-ccd-romk-current-block","predicate":"reduces-native-current-through","statement":"Mg2+ supplied through the whole-cell pipette reduced tertiapin-Q-sensitive current in rat cortical collecting-duct principal cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b58cb8e1-f9ff-5bb7-9dc1-61fe1fb35f23","mechanism_event_label":"Native kidney-cell recordings support the Mg brake on ROMK potassium flow seen in expressed channels.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"bdc516b4-647c-52b3-9b85-c925a0aec2ce","slug":"kcnj1","display_name":"Renal outer medullary potassium channel / KCNJ1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b58cb8e1-f9ff-5bb7-9dc1-61fe1fb35f23","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-native-ccd-romk-current-block-event","event_type":"biochemical_relationship","label":"Native kidney-cell recordings support the Mg brake on ROMK potassium flow seen in expressed channels.","description":"Mg2+ supplied through the whole-cell pipette reduced tertiapin-Q-sensitive current in rat cortical collecting-duct principal cells.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"current carrier","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bdc516b4-647c-52b3-9b85-c925a0aec2ce","slug":"kcnj1","display_name":"Renal outer medullary potassium channel / KCNJ1","entity_type_key":"protein"},"role":"tertiapin-Q-sensitive native channel","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"magnesium -> potassium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Whole-cell patch-clamp with controlled pipette Mg","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pipette-controlled Mg and voltage are not measurements of intracellular Mg during a human deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Native kidney-cell recordings support the Mg brake on ROMK potassium flow seen in expressed channels.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[yang-2010-romk-magnesium] Magnesium modulates ROMK channel-mediated potassium secretion (2010). https://pubmed.ncbi.nlm.nih.gov/21030597/ DOI: 10.1681/ASN.2010060617","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cortical collecting-duct principal cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"768aac66-a46d-5a5e-9396-87d18eeb91d5","evidence_kind":"source_excerpt","locator":"Lines 129-139","start_line":129,"end_line":139,"excerpt":"### mg-native-ccd-romk-current-block\nMg2+ supplied through the whole-cell pipette reduced tertiapin-Q-sensitive current in rat cortical collecting-duct principal cells.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Native kidney-cell recordings support the Mg brake on ROMK potassium flow seen in expressed channels.\norganism: Rattus norvegicus\ntissue_or_cell_type: Cortical collecting-duct principal cells\nexperimental_model: Whole-cell patch-clamp with controlled pipette Mg\nlimitations: Pipette-controlled Mg and voltage are not measurements of intracellular Mg during a human deficiency.\ncross_nutrient: magnesium -> potassium\n[yang-2010-romk-magnesium] Magnesium modulates ROMK channel-mediated potassium secretion (2010). https://pubmed.ncbi.nlm.nih.gov/21030597/ DOI: 10.1681/ASN.2010060617","model_system":"Whole-cell patch-clamp with controlled pipette Mg","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [yang-2010-romk-magnesium] Magnesium modulates ROMK channel-mediated potassium secretion (2010). https://pubmed.ncbi.nlm.nih.gov/21030597/ DOI: 10.1681/ASN.2010060617","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}