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Cellular enzyme activity does not prove clinical cancer benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Curcumin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A cultured human-cell assay also showed enzyme inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[curcumin-p15879598] Thioredoxin reductase is irreversibly modified by curcumin: a novel molecular mechanism for its anticancer activity. (2005). https://pubmed.ncbi.nlm.nih.gov/15879598/ DOI: 10.1074/jbc.m414645200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HeLa cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dcd550c0-e447-5a41-918a-33b3ca94c339","evidence_kind":"source_excerpt","locator":"Lines 541-552","start_line":541,"end_line":552,"excerpt":"### curcumin-hela-trx\nCurcumin inhibited measured TrxR activity in cultured HeLa cells at about 15 micromolar IC50.\nCondition category: normal\nnutrient_topic: Curcumin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A cultured human-cell assay also showed enzyme inhibition.\norganism: Homo sapiens\ntissue_or_cell_type: HeLa cells\nexperimental_model: Purified rat enzyme and separate HeLa-cell assays\nlimitations: Rat residue numbering Cys496/Sec497 is source-specific and must not replace numbering in other TrxR constructs. Cellular enzyme activity does not prove clinical cancer benefit.\nexposure: Rat enzyme: two-hour incubation, IC50 3.6 micromolar; HeLa activity IC50 about 15 micromolar\nevidence_span: {\"source_cache\": \"artifacts/curcumin-research/15879598.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"be68f9ed2b0741db356a6bc020304fe832121f92dfd9c423ce8a305e984e995c\", \"start_char\": 0, \"end_char\": 1659, \"text_sha256\": \"be68f9ed2b0741db356a6bc020304fe832121f92dfd9c423ce8a305e984e995c\"}\n[curcumin-p15879598] Thioredoxin reductase is irreversibly modified by curcumin: a novel molecular mechanism for its anticancer activity. 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