{"id":"9a6cc9ee-20b7-52c5-860b-7f5c0fb7cb12","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-tet1-recruitment","predicate":"increases_promoter_association","statement":"Luteolin increased TET1 association with the NFE2L2 promoter in human colon cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"316e2402-414f-5336-9431-e308ea906ed3","mechanism_event_label":"DNA-modifying machinery participates in the response.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"5fcc7eb2-480f-576e-9028-cb5f8f8a6d73","slug":"tet1","display_name":"TET1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"316e2402-414f-5336-9431-e308ea906ed3","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-tet1-recruitment-event","event_type":"observed_relationship","label":"DNA-modifying machinery participates in the response.","description":"Luteolin increased TET1 association with the NFE2L2 promoter in human colon cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5fcc7eb2-480f-576e-9028-cb5f8f8a6d73","slug":"tet1","display_name":"TET1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell ChIP; 10–60 micromolar studies.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Promoter association is not a direct luteolin–TET1 binding assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"DNA-modifying machinery participates in the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin promotes apoptotic cell death via upregulation of Nrf2 expression by DNA demethylase and the interaction of Nrf2 with p53 in human colon cancer cells. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30988303/ · DOI 10.1038/s12276-019-0238-y","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"589f9f30-9132-501e-8837-2d760d353761","evidence_kind":"source_excerpt","locator":"Lines 484-490","start_line":484,"end_line":490,"excerpt":"## luteolin-tet1-recruitment\nDNA-modifying machinery participates in the response.\nLuteolin increased TET1 association with the NFE2L2 promoter in human colon cancer cells.\nModel: Human cell ChIP; 10–60 micromolar studies.\nLimitations: Promoter association is not a direct luteolin–TET1 binding assay.\nEvidence access: Primary full text\nLuteolin promotes apoptotic cell death via upregulation of Nrf2 expression by DNA demethylase and the interaction of Nrf2 with p53 in human colon cancer cells. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30988303/ · DOI 10.1038/s12276-019-0238-y","model_system":"Human cell ChIP; 10–60 micromolar studies.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}