{"id":"9a118606-ef0e-5022-bc86-feeea62eca2d","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-enterococcus-reduction","predicate":"undergoes_bacterial_decolorization","statement":"Human-gut E. faecalis isolate AZO-Ef decolorized tartrazine in the substrate-panel experiment.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fd58c211-4d3c-5287-a7af-325d9d0cccd6","mechanism_event_label":"Human-gut E. faecalis isolate AZO-Ef decolorized tartrazine in the substrate-panel experiment.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"e1710d84-fd20-5710-848e-dae533d1ad34","slug":"enterococcus-faecalis-azo-ef-dye-reduction","display_name":"Tartrazine decolorization by human-gut E. faecalis isolate AZO-Ef","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fd58c211-4d3c-5287-a7af-325d9d0cccd6","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-enterococcus-reduction-event","event_type":"observed_relationship","label":"Human-gut E. faecalis isolate AZO-Ef decolorized tartrazine in the substrate-panel experiment.","description":"Human-gut E. faecalis isolate AZO-Ef decolorized tartrazine in the substrate-panel experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e1710d84-fd20-5710-848e-dae533d1ad34","slug":"enterococcus-faecalis-azo-ef-dye-reduction","display_name":"Tartrazine decolorization by human-gut E. faecalis isolate AZO-Ef","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 10, 20 and 30 micromolar; selected strains","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"30 min comparison, time courses up to 5 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacterial isolates from healthy adult human stool: E. coli AZO-Ec and E. faecalis AZO-Ef","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Decolorization is an activity readout, not a complete product mass balance or a clinical microbiome intervention. Strain, pH and oxygen dependence preclude assigning one universal rate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Bacterial isolates from healthy adult human stool: E. coli AZO-Ec and E. faecalis AZO-Ef","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human-gut E. faecalis isolate AZO-Ef decolorized tartrazine in the substrate-panel experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Azoreductase activity of dye-decolorizing bacteria isolated from the human gut microbiota. (2019). https://pubmed.ncbi.nlm.nih.gov/30940826/ DOI: 10.1038/s41598-019-41894-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro culture exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Aerobic bacterial culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"48c1cff8-b381-585f-81f7-ee10f3d9442f","evidence_kind":"source_excerpt","locator":"Lines 72-81","start_line":72,"end_line":81,"excerpt":"## tartrazine-enterococcus-reduction\nHuman-gut E. faecalis isolate AZO-Ef decolorized tartrazine in the substrate-panel experiment.\nModel/species: Bacterial isolates from healthy adult human stool: E. coli AZO-Ec and E. faecalis AZO-Ef\nTissue: Aerobic bacterial culture\nExposure: Tartrazine 10, 20 and 30 micromolar; selected strains\nRoute: In vitro culture exposure\nDuration: 30 min comparison, time courses up to 5 h\nLimits: Decolorization is an activity readout, not a complete product mass balance or a clinical microbiome intervention. Strain, pH and oxygen dependence preclude assigning one universal rate.\nPrimary reference: Azoreductase activity of dye-decolorizing bacteria isolated from the human gut microbiota. (2019). https://pubmed.ncbi.nlm.nih.gov/30940826/ DOI: 10.1038/s41598-019-41894-8\nAccess: Primary full-text methods/results and metadata.","model_system":"Bacterial isolates from healthy adult human stool: E. coli AZO-Ec and E. faecalis AZO-Ef","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}