{"id":"9a0b3864-6804-5c4a-b007-ff08b3070ade","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-lowers-citrate-excretion","predicate":"depletion-decreases","statement":"The potassium-depleted rats had reduced fractional urinary citrate excretion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ef62ac20-4114-5884-8417-57c86f8d7682","mechanism_event_label":"Less filtered citrate remained in urine during depletion.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"73f00d50-d3c9-5b8e-8e38-a5d004da46b7","slug":"urinary-citrate-excretion","display_name":"Urinary citrate excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ef62ac20-4114-5884-8417-57c86f8d7682","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-depletion-lowers-citrate-excretion-event","event_type":"biochemical_relationship","label":"Less filtered citrate remained in urine during depletion.","description":"The potassium-depleted rats had reduced fractional urinary citrate excretion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"73f00d50-d3c9-5b8e-8e38-a5d004da46b7","slug":"urinary-citrate-excretion","display_name":"Urinary citrate excretion","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1c54abac-4dfd-5303-b0f4-37945245e18e","slug":"citrate","display_name":"Citrate","entity_type_key":"small_molecule"},"role":"measured-anion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"potential-urinary-interaction-partner","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Citrate affects urinary calcium chemistry, but this depletion experiment measured citrate handling rather than stones.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"The potassium-depleted rats had reduced fractional urinary citrate excretion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Rats given low-potassium diet for 14 days; urine citrate and renal cortical brush-border membrane vesicle transport kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats given low-potassium diet for 14 days; urine citrate and renal cortical brush-border membrane vesicle transport kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The transport and excretion measurements were parallel; downstream stone formation was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Less filtered citrate remained in urine during depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[levi-1991-citrate] Chronic K depletion stimulates rat renal brush-border membrane Na-citrate cotransporter (1991). https://pubmed.ncbi.nlm.nih.gov/1683169/ DOI: 10.1152/ajprenal.1991.261.5.F767","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"kidney and urine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"92ea3ef9-3c96-588f-92c4-f82a572d5124","evidence_kind":"source_excerpt","locator":"Lines 1204-1216","start_line":1204,"end_line":1216,"excerpt":"### k-depletion-lowers-citrate-excretion\nThe potassium-depleted rats had reduced fractional urinary citrate excretion.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Less filtered citrate remained in urine during depletion.\norganism: Rattus norvegicus\ntissue_or_cell_type: kidney and urine\nexperimental_model: Rats given low-potassium diet for 14 days; urine citrate and renal cortical brush-border membrane vesicle transport kinetics.\nlimitations: The transport and excretion measurements were parallel; downstream stone formation was not tested.\ncross_nutrient: Citrate affects urinary calcium chemistry, but this depletion experiment measured citrate handling rather than stones.\nexperimental-exposure: Rats given low-potassium diet for 14 days; urine citrate and renal cortical brush-border membrane vesicle transport kinetics.\nendpoint: The potassium-depleted rats had reduced fractional urinary citrate excretion.\n[levi-1991-citrate] Chronic K depletion stimulates rat renal brush-border membrane Na-citrate cotransporter (1991). https://pubmed.ncbi.nlm.nih.gov/1683169/ DOI: 10.1152/ajprenal.1991.261.5.F767","model_system":"Rats given low-potassium diet for 14 days; urine citrate and renal cortical brush-border membrane vesicle transport kinetics.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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