{"id":"99cc274d-4f1f-5221-8851-7407d21d5586","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ifng-ido-depletion","predicate":"induces_catabolism_via","statement":"IFN-gamma induced IDO activity and depleted almost all culture-medium tryptophan over two to three days in responsive human KB and WiDr cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"14698117-7552-5540-b8e0-bd9c8c6728b5","mechanism_event_label":"An immune signal can locally consume the available amino acid.","subject":{"id":"393e7fb8-8b09-5b6a-b7e4-a31a78b4fcfb","slug":"ifng","display_name":"Human interferon gamma / IFNG","entity_type_key":"cytokine"},"object":{"id":"9edba4aa-c2f5-5a4e-9750-15e9a38b1822","slug":"ido1","display_name":"Human indoleamine 2,3-dioxygenase 1 / IDO1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"14698117-7552-5540-b8e0-bd9c8c6728b5","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ifng-ido-depletion-event","event_type":"observed_relationship","label":"An immune signal can locally consume the available amino acid.","description":"IFN-gamma induced IDO activity and depleted almost all culture-medium tryptophan over two to three days in responsive human KB and WiDr cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"393e7fb8-8b09-5b6a-b7e4-a31a78b4fcfb","slug":"ifng","display_name":"Human interferon gamma / IFNG","entity_type_key":"cytokine"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9edba4aa-c2f5-5a4e-9750-15e9a38b1822","slug":"ido1","display_name":"Human indoleamine 2,3-dioxygenase 1 / IDO1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cancer-cell cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Other tested cell lines lacked this induced enzyme response; not a universal effect of every interferon exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An immune signal can locally consume the available amino acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma. · 1988 · https://pubmed.ncbi.nlm.nih.gov/3124115/ · DOI 10.1073/pnas.85.4.1242","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b2334b30-01e4-5be0-b98c-734f227ae496","evidence_kind":"source_excerpt","locator":"Lines 322-328","start_line":322,"end_line":328,"excerpt":"## tryptophan-ifng-ido-depletion\nAn immune signal can locally consume the available amino acid.\nIFN-gamma induced IDO activity and depleted almost all culture-medium tryptophan over two to three days in responsive human KB and WiDr cells.\nModel: Human cancer-cell cultures.\nLimitations: Other tested cell lines lacked this induced enzyme response; not a universal effect of every interferon exposure.\nEvidence access: Primary abstract\nInduction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma. · 1988 · https://pubmed.ncbi.nlm.nih.gov/3124115/ · DOI 10.1073/pnas.85.4.1242","model_system":"Human cancer-cell cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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