{"id":"99b76b0d-d297-57b1-9516-c42d01ecdf26","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-esp-nitrile","predicate":"redirects_hydrolysis_toward","statement":"Recombinant broccoli ESP redirected myrosinase-dependent glucoraphanin hydrolysis toward sulforaphane nitrile rather than sulforaphane.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7a5e5b88-7676-5904-9548-eb5fde0612a0","mechanism_event_label":"A second plant protein changes which molecule is produced.","subject":{"id":"38c3086b-4fbf-571f-8da8-6baaa8527333","slug":"broccoli-esp","display_name":"Broccoli epithiospecifier protein / ESP","entity_type_key":"protein"},"object":{"id":"ce51acba-6caf-5d46-bc3d-8803fca880d8","slug":"sulforaphane-nitrile","display_name":"Sulforaphane nitrile","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7a5e5b88-7676-5904-9548-eb5fde0612a0","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-esp-nitrile-event","event_type":"biochemical_relationship","label":"A second plant protein changes which molecule is produced.","description":"Recombinant broccoli ESP redirected myrosinase-dependent glucoraphanin hydrolysis toward sulforaphane nitrile rather than sulforaphane.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"00d3016d-ac2d-58a7-86b1-3052bd4239fe","slug":"glucoraphanin","display_name":"Glucoraphanin","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8b40a993-546c-5d0d-91f9-3886604a30a8","slug":"broccoli-myrosinase","display_name":"Broccoli myrosinase, study-resolved enzyme","entity_type_key":"protein"},"role":"hydrolase","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"alternative_product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"38c3086b-4fbf-571f-8da8-6baaa8527333","slug":"broccoli-esp","display_name":"Broccoli epithiospecifier protein / ESP","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ce51acba-6caf-5d46-bc3d-8803fca880d8","slug":"sulforaphane-nitrile","display_name":"Sulforaphane nitrile","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/16536577.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e3636614520a0c9f83132284992ffc10ca42943f97619933f8009ceb697ddfa9\", \"start_char\": 0, \"end_char\": 1301, \"text_sha256\": \"e3636614520a0c9f83132284992ffc10ca42943f97619933f8009ceb697ddfa9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant protein and glucosinolate product analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified recombinant ESP in a model hydrolysis system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Plant chemistry; does not establish a human health effect of the nitrile product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Broccoli cv. Packman ESP expressed in E. coli","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second plant protein changes which molecule is produced.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p16536577] Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane. (2006). https://pubmed.ncbi.nlm.nih.gov/16536577/ DOI: 10.1021/jf0525277","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Myrosinase-dependent glucoraphanin conversion","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"359d325d-9cb1-52e1-80df-5436d3ce4af7","evidence_kind":"source_excerpt","locator":"Lines 177-188","start_line":177,"end_line":188,"excerpt":"### sulforaphane-esp-nitrile\nRecombinant broccoli ESP redirected myrosinase-dependent glucoraphanin hydrolysis toward sulforaphane nitrile rather than sulforaphane.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second plant protein changes which molecule is produced.\norganism: Broccoli cv. Packman ESP expressed in E. coli\ntissue_or_cell_type: Myrosinase-dependent glucoraphanin conversion\nexperimental_model: Recombinant protein and glucosinolate product analysis\nlimitations: Plant chemistry; does not establish a human health effect of the nitrile product.\nexposure: Purified recombinant ESP in a model hydrolysis system\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/16536577.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e3636614520a0c9f83132284992ffc10ca42943f97619933f8009ceb697ddfa9\", \"start_char\": 0, \"end_char\": 1301, \"text_sha256\": \"e3636614520a0c9f83132284992ffc10ca42943f97619933f8009ceb697ddfa9\"}\n[sulforaphane-p16536577] Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane. (2006). https://pubmed.ncbi.nlm.nih.gov/16536577/ DOI: 10.1021/jf0525277","model_system":"Recombinant protein and glucosinolate product analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p16536577] Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane. (2006). https://pubmed.ncbi.nlm.nih.gov/16536577/ DOI: 10.1021/jf0525277","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"a4bafbf7-f117-53c9-a6d7-8fb25e4aca72","stable_key":"import-44eaeae5-557a-552b-8998-884f30462e2a","title":"Sulforaphane: formation, electrophile sensing and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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