{"id":"99a852e3-e4a9-5b40-973e-2586f1a3c092","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-uptake-oct2","predicate":"transports","statement":"Human OCT2 transported berberine in transfected cells, with reported Km 4.4 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aeab42da-a5c2-57af-bb03-9277897bc2b0","mechanism_event_label":"Berberine uses a separately identified organic-cation uptake transporter.","subject":{"id":"3617021d-d2f4-5199-80d7-c881b135621a","slug":"slc22a2","display_name":"Human organic cation transporter 2 / OCT2 / SLC22A2","entity_type_key":"protein"},"object":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"aeab42da-a5c2-57af-bb03-9277897bc2b0","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-uptake-oct2-event","event_type":"biochemical_relationship","label":"Berberine uses a separately identified organic-cation uptake transporter.","description":"Human OCT2 transported berberine in transfected cells, with reported Km 4.4 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3617021d-d2f4-5199-80d7-c881b135621a","slug":"slc22a2","display_name":"Human organic cation transporter 2 / OCT2 / SLC22A2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/berberine-research/18157518.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"34651ed2e9ce3c38b6042e616b5c36d932fe6f1099c627d1b5403b338eae7b2b\", \"start_char\": 0, \"end_char\": 1918, \"text_sha256\": \"34651ed2e9ce3c38b6042e616b5c36d932fe6f1099c627d1b5403b338eae7b2b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Polarized transporter-transfected MDCKII cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Berberine transport kinetics and transporter inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered system, not direct evidence for human oral bioavailability or a universal transport bottleneck.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Berberine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human OCT1, OCT2 and ABCB1 in engineered canine cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Berberine uses a separately identified organic-cation uptake transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[berberine-p18157518] Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1). (2008). https://pubmed.ncbi.nlm.nih.gov/18157518/ DOI: 10.1007/s00210-007-0219-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Basolateral uptake and apical export","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Berberine uptake measured in transfected cells, which stand for the tubular cell, with a reported Km of 4.4 micromolar.","entity":null},{"dimension":"transport_pool","value_text":"the renal tubular cell interior","comparator":null,"unit":null,"notes":"Berberine uptake measured in transfected cells, which stand for the tubular cell, with a reported Km of 4.4 micromolar.","entity":null}],"evidence":[{"id":"b18fe913-65c9-5112-aaad-1ae0860473dd","evidence_kind":"source_excerpt","locator":"Lines 220-231","start_line":220,"end_line":231,"excerpt":"### berberine-uptake-oct2\nHuman OCT2 transported berberine in transfected cells, with reported Km 4.4 micromolar.\nCondition category: normal\nnutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Berberine uses a separately identified organic-cation uptake transporter.\norganism: Human OCT1, OCT2 and ABCB1 in engineered canine cells\ntissue_or_cell_type: Basolateral uptake and apical export\nexperimental_model: Polarized transporter-transfected MDCKII cells\nlimitations: Engineered system, not direct evidence for human oral bioavailability or a universal transport bottleneck.\nexposure: Berberine transport kinetics and transporter inhibition\nevidence_span: {\"source_cache\": \"artifacts/berberine-research/18157518.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"34651ed2e9ce3c38b6042e616b5c36d932fe6f1099c627d1b5403b338eae7b2b\", \"start_char\": 0, \"end_char\": 1918, \"text_sha256\": \"34651ed2e9ce3c38b6042e616b5c36d932fe6f1099c627d1b5403b338eae7b2b\"}\n[berberine-p18157518] Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1). (2008). https://pubmed.ncbi.nlm.nih.gov/18157518/ DOI: 10.1007/s00210-007-0219-x","model_system":"Polarized transporter-transfected MDCKII cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [berberine-p18157518] Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1). 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