{"id":"999e4819-ffb9-52e4-ba8f-16f71ee081cf","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-alpha7-nicotinic-potentiation","predicate":"increases_activity_of","statement":"Preapplication of ivermectin in the micromolar range strongly enhanced the acetylcholine-evoked current of alpha7 nicotinic acetylcholine receptors, acting as a positive allosteric effector.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"99d16fbf-7682-51c5-82d3-b8aad0b51416","mechanism_event_label":"Preapplication of ivermectin in the micromolar range strongly enhanced the acetylcholine-evoked current of alpha7 nicotinic acetylcholine receptors, acting as a positive allosteric effector.","subject":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"object":{"id":"bee844eb-c0ae-57f9-ad5f-ead8a04ce4a6","slug":"chrna7","display_name":"Human alpha7 nicotinic acetylcholine receptor / CHRNA7","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"99d16fbf-7682-51c5-82d3-b8aad0b51416","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-alpha7-nicotinic-potentiation-event","event_type":"observed_relationship","label":"Preapplication of ivermectin in the micromolar range strongly enhanced the acetylcholine-evoked current of alpha7 nicotinic acetylcholine receptors, acting as a positive allosteric effector.","description":"Preapplication of ivermectin in the micromolar range strongly enhanced the acetylcholine-evoked current of alpha7 nicotinic acetylcholine receptors, acting as a positive allosteric effector.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bee844eb-c0ae-57f9-ad5f-ead8a04ce4a6","slug":"chrna7","display_name":"Human alpha7 nicotinic acetylcholine receptor / CHRNA7","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chick and human alpha7 nicotinic acetylcholine receptors in Xenopus laevis oocytes and K-28 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Micromolar ivermectin preapplication","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Micromolar exposure in a heterologous expression system is well above ordinary antiparasitic plasma concentrations, and the authors exclude a nonspecific chloride current rather than establishing an in vivo effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Chick and human alpha7 nicotinic acetylcholine receptors in Xenopus laevis oocytes and K-28 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Preapplication of ivermectin in the micromolar range strongly enhanced the acetylcholine-evoked current of alpha7 nicotinic acetylcholine receptors, acting as a positive allosteric effector.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ivermectin: a positive allosteric effector of the alpha7 neuronal nicotinic acetylcholine receptor. (1998). https://pubmed.ncbi.nlm.nih.gov/9463487/ DOI: 10.1124/mol.53.2.283","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Ligand-gated cation channel current","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5f39752c-b2af-5a84-b20d-336e226d48a3","evidence_kind":"source_excerpt","locator":"Lines 79-88","start_line":79,"end_line":88,"excerpt":"## ivermectin-alpha7-nicotinic-potentiation\nPreapplication of ivermectin in the micromolar range strongly enhanced the acetylcholine-evoked current of alpha7 nicotinic acetylcholine receptors, acting as a positive allosteric effector.\nModel/species: Chick and human alpha7 nicotinic acetylcholine receptors in Xenopus laevis oocytes and K-28 cells\nTissue/system: Ligand-gated cation channel current\nExposure: Micromolar ivermectin preapplication\nRoute: In vitro\nDuration: Acute\nLimits: Micromolar exposure in a heterologous expression system is well above ordinary antiparasitic plasma concentrations, and the authors exclude a nonspecific chloride current rather than establishing an in vivo effect.\nPrimary reference: Ivermectin: a positive allosteric effector of the alpha7 neuronal nicotinic acetylcholine receptor. (1998). https://pubmed.ncbi.nlm.nih.gov/9463487/ DOI: 10.1124/mol.53.2.283\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"20818ddd-7b37-5226-a24b-7de007e80eb2","stable_key":"import-e0801c92-7fd3-5cb2-82b7-dd82f010f3ed","title":"Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"c7e1e0201cbd222f0c52b7981f078936ed39b617d747821ed6daa584c2b964a7","revision_id":"56746c3e-cdec-5b8f-a191-425de1a0a7ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}