{"id":"99997a22-5259-5be9-8d38-e68af887c484","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-brain-mda-null","predicate":"no_significant_change","statement":"Tartrazine alone did not significantly change the brain MDA assay endpoint in this two-week rat study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e119cb9e-f726-557a-ad15-f09e88866c98","mechanism_event_label":"Tartrazine alone did not significantly change the brain MDA assay endpoint in this two-week rat study.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"e60ea03f-d0d7-5818-b743-12f9ebddf717","slug":"rat-brain-mda-assay","display_name":"Rat brain malondialdehyde assay signal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e119cb9e-f726-557a-ad15-f09e88866c98","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-brain-mda-null-event","event_type":"observed_relationship","label":"Tartrazine alone did not significantly change the brain MDA assay endpoint in this two-week rat study.","description":"Tartrazine alone did not significantly change the brain MDA assay endpoint in this two-week rat study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e60ea03f-d0d7-5818-b743-12f9ebddf717","slug":"rat-brain-mda-assay","display_name":"Rat brain malondialdehyde assay signal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 700 mg/kg body weight for 2 weeks","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"2 treatment weeks; endpoint tissue analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male Wistar rats; six per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Very high animal exposure. Results section reports lower ascorbic acid with no significant GSH or MDA change from tartrazine alone; broader abstract/discussion wording should not replace these results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Male Wistar rats; six per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine alone did not significantly change the brain MDA assay endpoint in this two-week rat study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"High-Dose Aspirin Reverses Tartrazine-Induced Cell Growth Dysregulation Independent of p53 Signaling and Antioxidant Mechanisms in Rat Brain. (2019). https://pubmed.ncbi.nlm.nih.gov/31032366/ DOI: 10.1155/2019/9096404","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Oral tartrazine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Brain tissue biochemical and histological endpoints","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fef485d4-6623-59de-a690-fe8a2330f00b","evidence_kind":"source_excerpt","locator":"Lines 380-389","start_line":380,"end_line":389,"excerpt":"## tartrazine-brain-mda-null\nTartrazine alone did not significantly change the brain MDA assay endpoint in this two-week rat study.\nModel/species: Male Wistar rats; six per group\nTissue: Brain tissue biochemical and histological endpoints\nExposure: Tartrazine 700 mg/kg body weight for 2 weeks\nRoute: Oral tartrazine\nDuration: 2 treatment weeks; endpoint tissue analysis\nLimits: Very high animal exposure. Results section reports lower ascorbic acid with no significant GSH or MDA change from tartrazine alone; broader abstract/discussion wording should not replace these results.\nPrimary reference: High-Dose Aspirin Reverses Tartrazine-Induced Cell Growth Dysregulation Independent of p53 Signaling and Antioxidant Mechanisms in Rat Brain. (2019). https://pubmed.ncbi.nlm.nih.gov/31032366/ DOI: 10.1155/2019/9096404\nAccess: Primary full-text methods/results and metadata.","model_system":"Male Wistar rats; six per group","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. 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