{"id":"995771c0-7882-5fc4-8603-85243cd71776","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-bovine-no","predicate":"increases_tested","statement":"SAC increased the NO-release readout in BAE-1 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a6f8819f-3ce9-5724-b3e5-d78a0fd992f0","mechanism_event_label":"The downstream gas signal was measured separately.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a6f8819f-3ce9-5724-b3e5-d78a0fd992f0","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-bovine-no-event","event_type":"observed_relationship","label":"The downstream gas signal was measured separately.","description":"SAC increased the NO-release readout in BAE-1 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f2a6b7ab-ad41-5ada-8895-41d6ce42f4e4","slug":"nitric-oxide","display_name":"NO","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"201a30a0-80c2-5a25-8be8-b75bc9df7164","slug":"bovine-nos3","display_name":"Bovine endothelial nitric oxide synthase / NOS3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"Purified SAC and black garlic extract tested separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bovine aortic endothelial BAE-1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not proof of lowered human blood pressure or independence from arginine and NOS cofactors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Bos taurus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The downstream gas signal was measured separately.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[37062967] Improving endothelial health with food-derived H2S donors: an in vitro study with S-allyl cysteine and with a black-garlic extract enriched in sulfur-containing compounds. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37062967/ · DOI 10.1039/d3fo00412k","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Bovine aortic endothelial BAE-1 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"490c7023-dabf-501f-8024-80cf59833b18","evidence_kind":"source_excerpt","locator":"Lines 284-291","start_line":284,"end_line":291,"excerpt":"## s-allylcysteine-bovine-no\nThe downstream gas signal was measured separately.\nSAC increased the NO-release readout in BAE-1 cells.\nModel: Bovine aortic endothelial BAE-1 cells\nLimitations: Not proof of lowered human blood pressure or independence from arginine and NOS cofactors.\nEvidence access: Primary abstract\n[37062967] Improving endothelial health with food-derived H2S donors: an in vitro study with S-allyl cysteine and with a black-garlic extract enriched in sulfur-containing compounds. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37062967/ · DOI 10.1039/d3fo00412k\nStructured context: {\"organism\": \"Bos taurus\", \"tissue_or_cell_type\": \"Bovine aortic endothelial BAE-1 cells\", \"dose\": \"Not specified in accessed abstract\", \"duration\": \"Not specified in accessed abstract\", \"route\": \"Cell culture\", \"experimental_comparison\": \"Purified SAC and black garlic extract tested separately\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Bovine aortic endothelial BAE-1 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"91d7aeac82e42ce559d13dd8a2e0165d37760f7f555dd01c80e0604fa6e71ea2","revision_id":"545e497c-e5e3-543c-b26b-b297cbc7bbec","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}