{"id":"994c0e65-7f0a-5700-afbf-d0325d0cace2","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmaa-reactivates-mmut","predicate":"reactivates","statement":"Adding MMAA after 60 minutes restored activity in an inactivated human MMUT reaction through GTP hydrolysis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"95b4e0b8-f4fc-5911-b999-913311ec6f04","mechanism_event_label":"MMAA restarted an inactive purified human MMUT reaction in a GTP-dependent assay.","subject":{"id":"39366dce-a30d-5e74-8c86-2bd8ca0bad96","slug":"mmaa","display_name":"Human MMAA GTPase","entity_type_key":"protein"},"object":{"id":"8f690797-c976-5a5e-886e-dc49d1b17f5a","slug":"mmut","display_name":"Human methylmalonyl-CoA mutase / MMUT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"95b4e0b8-f4fc-5911-b999-913311ec6f04","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmaa-reactivates-mmut-event","event_type":"biochemical_relationship","label":"MMAA restarted an inactive purified human MMUT reaction in a GTP-dependent assay.","description":"Adding MMAA after 60 minutes restored activity in an inactivated human MMUT reaction through GTP hydrolysis.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"c8ccc03a-29cc-5127-baf5-81eeb92ac780","slug":"gtp","display_name":"Guanosine triphosphate","entity_type_key":"small_molecule"},"role":"energy-substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07657685-74aa-5d99-8625-0f6a3b521f07","slug":"adenosylcobalamin","display_name":"5'-Deoxyadenosylcobalamin","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"39366dce-a30d-5e74-8c86-2bd8ca0bad96","slug":"mmaa","display_name":"Human MMAA GTPase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8f690797-c976-5a5e-886e-dc49d1b17f5a","slug":"mmut","display_name":"Human methylmalonyl-CoA mutase / MMUT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Indexed primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"MMAA added after 60-minute catalytic inactivation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In-vitro reactivation; abstract interprets damaged-cofactor exchange rather than measuring every intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MMAA restarted an inactive purified human MMUT reaction in a GTP-dependent assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[takahashi-2010-mmaa-repair] Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein. (2011). https://pubmed.ncbi.nlm.nih.gov/21138732/ DOI: 10.1016/j.bbrc.2010.11.141","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9377bdd4-210a-5705-abeb-9650fcd3cd3a","evidence_kind":"source_excerpt","locator":"Lines 1089-1101","start_line":1089,"end_line":1101,"excerpt":"### mmaa-reactivates-mmut\nAdding MMAA after 60 minutes restored activity in an inactivated human MMUT reaction through GTP hydrolysis.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: MMAA restarted an inactive purified human MMUT reaction in a GTP-dependent assay.\norganism: Homo sapiens\ntissue_or_cell_type: Purified protein assay\nexperimental_model: Purified human proteins\nlimitations: In-vitro reactivation; abstract interprets damaged-cofactor exchange rather than measuring every intermediate.\nexposure: MMAA added after 60-minute catalytic inactivation\ncross_nutrient: false\nevidence_location: Indexed primary abstract\n[takahashi-2010-mmaa-repair] Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein. 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