{"id":"993e0baa-44bc-5c63-b4b4-9f297bc9247e","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-muscle-myotubes","predicate":"increases","statement":"A 0.1 mM silicon preparation increased C2C12 viability, migration and myotube formation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"62c063a8-3c38-5251-93aa-290c81c81b66","mechanism_event_label":"The soluble-preparation findings extend beyond bone cells.","subject":{"id":"36f2ccf3-5023-5074-aab3-d65d3650b9f6","slug":"metasilicate-derived-culture-solution","display_name":"Sodium-metasilicate-derived soluble silicon culture preparation","entity_type_key":"chemical_species"},"object":{"id":"e99b3d78-84a1-53f9-85d4-884ff3a1595b","slug":"mouse-c2c12-myotube-formation","display_name":"Myotube formation by mouse C2C12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"62c063a8-3c38-5251-93aa-290c81c81b66","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-muscle-myotubes-event","event_type":"observed_relationship","label":"The soluble-preparation findings extend beyond bone cells.","description":"A 0.1 mM silicon preparation increased C2C12 viability, migration and myotube formation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36f2ccf3-5023-5074-aab3-d65d3650b9f6","slug":"metasilicate-derived-culture-solution","display_name":"Sodium-metasilicate-derived soluble silicon culture preparation","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e99b3d78-84a1-53f9-85d4-884ff3a1595b","slug":"mouse-c2c12-myotube-formation","display_name":"Myotube formation by mouse C2C12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse C2C12 cultures; sodium-metasilicate-derived medium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not proof of human muscle regeneration after supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The soluble-preparation findings extend beyond bone cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ionic Silicon Protects Oxidative Damage and Promotes Skeletal Muscle Cell Regeneration. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33419056/ · DOI 10.3390/ijms22020497","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"704f7ff6-8659-5eb2-b6ce-8be6e3151e4e","evidence_kind":"source_excerpt","locator":"Lines 320-326","start_line":320,"end_line":326,"excerpt":"## silica-muscle-myotubes\nThe soluble-preparation findings extend beyond bone cells.\nA 0.1 mM silicon preparation increased C2C12 viability, migration and myotube formation.\nModel: Mouse C2C12 cultures; sodium-metasilicate-derived medium.\nLimitations: Not proof of human muscle regeneration after supplementation.\nEvidence access: Primary full text\nIonic Silicon Protects Oxidative Damage and Promotes Skeletal Muscle Cell Regeneration. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33419056/ · DOI 10.3390/ijms22020497","model_system":"Mouse C2C12 cultures; sodium-metasilicate-derived medium.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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