{"id":"992a76e4-408e-5491-bd56-ab693c52e22d","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-zinc-alpl-reconstitution","predicate":"supports_zinc_dependent_activity","statement":"Combined Mg and Zn reconstituted demetalated TNAP activity; neither metal alone restored the same activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6ea58e3c-1533-5309-bfe4-0554f6967392","mechanism_event_label":"This mineralization enzyme needs the right metals in different sites.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"3148c377-9b4c-56ca-bc36-081bf1905d57","slug":"alpl","display_name":"Tissue-nonspecific alkaline phosphatase ALPL / TNAP","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"6ea58e3c-1533-5309-bfe4-0554f6967392","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-zinc-alpl-reconstitution-event","event_type":"biochemical_relationship","label":"This mineralization enzyme needs the right metals in different sites.","description":"Combined Mg and Zn reconstituted demetalated TNAP activity; neither metal alone restored the same activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"catalytic_metal","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"role":"mineral_context","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3148c377-9b4c-56ca-bc36-081bf1905d57","slug":"alpl","display_name":"Tissue-nonspecific alkaline phosphatase ALPL / TNAP","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium + zinc -> ALPL; connects to calcium/phosphate mineralization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant TNAP metal-reconstitution assay. Activity was measured using artificial pNPP substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not an oral supplementation synergy trial. This assay does not quantify physiological pyrophosphate turnover or mineral deposition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This mineralization enzyme needs the right metals in different sites.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mg-hoylaerts2015] Functional significance of calcium binding to tissue-nonspecific alkaline phosphatase (2015). https://pubmed.ncbi.nlm.nih.gov/25775211/ DOI: 10.1371/journal.pone.0119874","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant human TNAP; cell-free p-nitrophenyl phosphate (pNPP) hydrolysis assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"505450f7-43e2-51d9-b87a-a9aa822a8d87","evidence_kind":"source_excerpt","locator":"Lines 1467-1477","start_line":1467,"end_line":1477,"excerpt":"### mg-zinc-alpl-reconstitution\nCombined Mg and Zn reconstituted demetalated TNAP activity; neither metal alone restored the same activity.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This mineralization enzyme needs the right metals in different sites.\norganism: Homo sapiens\ntissue_or_cell_type: Recombinant human TNAP; cell-free p-nitrophenyl phosphate (pNPP) hydrolysis assay\nexperimental_model: Recombinant TNAP metal-reconstitution assay. Activity was measured using artificial pNPP substrate.\nlimitations: Not an oral supplementation synergy trial. This assay does not quantify physiological pyrophosphate turnover or mineral deposition.\ncross_nutrient: Magnesium + zinc -> ALPL; connects to calcium/phosphate mineralization.\n[mg-hoylaerts2015] Functional significance of calcium binding to tissue-nonspecific alkaline phosphatase (2015). https://pubmed.ncbi.nlm.nih.gov/25775211/ DOI: 10.1371/journal.pone.0119874","model_system":"Recombinant TNAP metal-reconstitution assay. Activity was measured using artificial pNPP substrate.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mg-hoylaerts2015] Functional significance of calcium binding to tissue-nonspecific alkaline phosphatase (2015). https://pubmed.ncbi.nlm.nih.gov/25775211/ DOI: 10.1371/journal.pone.0119874","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}