{"id":"9890b29d-6723-53ee-9f55-d64c13642290","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-sda-assembly","predicate":"participates_in","statement":"The NFS1–ISD11–ACP core associates with ISCU, frataxin and ferredoxin to support Fe-S cluster biosynthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b9d51f54-63f9-5dd1-9866-14881cd9e5e7","mechanism_event_label":"Iron needs an assembly system and a sulfur supply before it becomes a working iron-sulfur cofactor.","subject":{"id":"9838e184-216a-5a26-a8c5-1777b9dd2d90","slug":"nfs1","display_name":"Human cysteine desulfurase / NFS1","entity_type_key":"protein"},"object":{"id":"34d475be-4832-5751-9aef-9c92a38254e7","slug":"iron-sulfur-cluster-assembly","display_name":"Iron-sulfur cluster biosynthesis and assembly","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b9d51f54-63f9-5dd1-9866-14881cd9e5e7","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-sda-assembly-event","event_type":"biochemical_relationship","label":"Iron needs an assembly system and a sulfur supply before it becomes a working iron-sulfur cofactor.","description":"The NFS1–ISD11–ACP core associates with ISCU, frataxin and ferredoxin to support Fe-S cluster biosynthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c78d3c3c-59b9-5192-b7ec-498124752c92","slug":"lyrm4","display_name":"Human ISD11 / LYRM4","entity_type_key":"protein"},"role":"assembly_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3474afb8-5d3d-5b1c-a8a6-a5450091b5c0","slug":"iscu","display_name":"Human ISCU","entity_type_key":"protein"},"role":"scaffold","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e6ec51dc-a24c-5019-9aaf-b2e47a755561","slug":"fxn","display_name":"Human frataxin / FXN","entity_type_key":"protein"},"role":"regulator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"sulfur_source","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9838e184-216a-5a26-a8c5-1777b9dd2d90","slug":"nfs1","display_name":"Human cysteine desulfurase / NFS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"34d475be-4832-5751-9aef-9c92a38254e7","slug":"iron-sulfur-cluster-assembly","display_name":"Iron-sulfur cluster biosynthesis and assembly","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/28634302.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\", \"start_char\": 0, \"end_char\": 1850, \"text_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystallography, electron microscopy, kinetics and cell studies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SDA-complex structural analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Hybrid structural system: bacterial ACP must not be silently labeled human NDUFAB1. Direct dietary B6/B5 effects were not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human NFS1/ISD11 with bacterial ACP in the recombinant structural complex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Iron needs an assembly system and a sulfur supply before it becomes a working iron-sulfur cofactor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p28634302] Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. (2017). https://pubmed.ncbi.nlm.nih.gov/28634302/ DOI: 10.1073/pnas.1702849114","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial Fe-S assembly machinery","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be837d5b-b643-53c7-ba9c-175468ec7568","evidence_kind":"source_excerpt","locator":"Lines 1161-1172","start_line":1161,"end_line":1172,"excerpt":"### iron-sda-assembly\nThe NFS1–ISD11–ACP core associates with ISCU, frataxin and ferredoxin to support Fe-S cluster biosynthesis.\nCondition category: normal\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Iron needs an assembly system and a sulfur supply before it becomes a working iron-sulfur cofactor.\norganism: Human NFS1/ISD11 with bacterial ACP in the recombinant structural complex\ntissue_or_cell_type: Mitochondrial Fe-S assembly machinery\nexperimental_model: Crystallography, electron microscopy, kinetics and cell studies\nlimitations: Hybrid structural system: bacterial ACP must not be silently labeled human NDUFAB1. Direct dietary B6/B5 effects were not tested.\nexposure: SDA-complex structural analysis\nevidence_span: {\"source_cache\": \"artifacts/iron-research/28634302.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\", \"start_char\": 0, \"end_char\": 1850, \"text_sha256\": \"82faf07d78311d063c1d990ebb922062650054be372fb324fa67020790c9fa04\"}\n[iron-p28634302] Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. (2017). https://pubmed.ncbi.nlm.nih.gov/28634302/ DOI: 10.1073/pnas.1702849114","model_system":"Crystallography, electron microscopy, kinetics and cell studies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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