{"id":"988e0db5-6466-58b8-9e3d-4028a2f8d473","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-ptp-edta-reversal","predicate":"reverses_vanadate_inhibition","statement":"Adding EDTA immediately and completely reversed vanadate inhibition of PTP1B in the assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"70710c10-48d0-5a35-9ed8-de164351ea6f","mechanism_event_label":"Removing available inhibitor restored activity in this preparation.","subject":{"id":"e55b9653-c097-5ca5-b8c3-f1dfd784247d","slug":"edta","display_name":"EDTA","entity_type_key":"small_molecule"},"object":{"id":"58af4c7b-a5f4-540c-85eb-28a6161d45e7","slug":"ptp1b-1997-assay-preparation","display_name":"PTP1B preparation in the 1997 vanadate inhibition study","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"70710c10-48d0-5a35-9ed8-de164351ea6f","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-ptp-edta-reversal-event","event_type":"observed_relationship","label":"Removing available inhibitor restored activity in this preparation.","description":"Adding EDTA immediately and completely reversed vanadate inhibition of PTP1B in the assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e55b9653-c097-5ca5-b8c3-f1dfd784247d","slug":"edta","display_name":"EDTA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58af4c7b-a5f4-540c-85eb-28a6161d45e7","slug":"ptp1b-1997-assay-preparation","display_name":"PTP1B preparation in the 1997 vanadate inhibition study","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Enzyme assay with a vanadate-chelating reagent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Biochemical reversal, not a clinical chelation treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Removing available inhibitor restored activity in this preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. · 1997 · https://pubmed.ncbi.nlm.nih.gov/8995372/ · DOI 10.1074/jbc.272.2.843","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b6e5b7ce-436d-5c09-b9f7-72fffde851e5","evidence_kind":"source_excerpt","locator":"Lines 22-28","start_line":22,"end_line":28,"excerpt":"## vanadium-ptp-edta-reversal\nRemoving available inhibitor restored activity in this preparation.\nAdding EDTA immediately and completely reversed vanadate inhibition of PTP1B in the assay.\nModel: Enzyme assay with a vanadate-chelating reagent.\nLimitations: Biochemical reversal, not a clinical chelation treatment.\nEvidence access: Primary abstract\nMechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. · 1997 · https://pubmed.ncbi.nlm.nih.gov/8995372/ · DOI 10.1074/jbc.272.2.843","model_system":"Enzyme assay with a vanadate-chelating reagent.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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